中国药理学通报2024，Vol.40Issue(4)：709-715,7.DOI:10.12360/CPB202311029
海马G蛋白偶联雌激素受体1参与癫痫调控的转录组学研究
Transcriptomic study of hippocampal G protein-coupled estrogen receptor 1 involved in epilepsy regulation
摘要
Abstract
Aim To focus on the RNA-Seq analysis of WT,Gper1-KD hippocampal tissues of rats and to seek the possible signal molecule and pathways in epilepto-genesis mediated by G-protein coupled estrogen recep-tor 1(GPER1).Methods RNA was extracted and cDNA library was constructed,then the reliable data were screened and blasted with rat genome from NCBI database.Then the DEGs were selected according to FPKM value.GO enrichment,KEGG enrichment and protein interaction network analysis were performed.Results On the grounds of screening conditions(FC≥1.5,P＜0.05)for DEGs,there were 2 253 genes selected between WT and Gper1-KD group animals,and 1 380 genes were significantly up-regulated and 873 down-regulated.The GO enrichment results re-vealed DEGs mainly played a part in the biological process including lymphocyte chemotaxis,secretion by cell,macrophage chemotaxis,neutrophil chemotaxis and positive regulation of angiogenesis.Moreover,the KEGG enrichment results demonstrated that the DEGs were mainly involved in pathways in cancer,PI3K-Akt pathway,proteoglycans in cancer,focal adhesion and MAPK pathways.RT-qPCR results showed that the ex-pression levels of Gper1,Foxo3,Camk2d,Pdpk1,Pik3cg,Mapk12 were significantly changed.Conclu-sions MAPK,TNF signaling pathway may play a key role in GPER1 affecting epileptogenesis,GABAergic neurons and Pik3cg may play an important role in GPER1 influencing epilepsy susceptibility.关键词
GPER1/癫痫/DEGs/GO富集/KEGG富集/蛋白互作网络Key words
GPER1/epilepsy/DEGs/GO enrich-ment/KEGG enrichment/protein interaction network分类
医药卫生引用本文复制引用
左娣,戎伟芳,刘昆梅,郝世杰,杨盼,李苗,任晓璠,丁娜,马文倩,王盼盼,王诗雨..海马G蛋白偶联雌激素受体1参与癫痫调控的转录组学研究[J].中国药理学通报,2024,40(4):709-715,7.基金项目
国家自然科学基金资助项目(No 82360269) （No 82360269）
宁夏重点研发项目(引才专项,No 2021BEB04017) （引才专项,No 2021BEB04017）
宁夏自然科学基金资助项目(No 2022AAC03189) （No 2022AAC03189）
大学生创新创业训练计划(No S202310752055) （No S202310752055）
