中国药理学通报2024，Vol.40Issue(4)：730-739,10.DOI:10.12360/CPB202309052
紫斑牡丹花粉对D-半乳糖致衰老模型小鼠的影响
Effects of Paeonia rockii pollen on D-galactose aging model mice
摘要
Abstract
Aim To investigate the protective effect of P.rockii pollen on the improvement of learning memo-ry and oxidative stress damage in D-galactose-induced aging mice.Methods Sixty C57BL/6 mice were ran-domly divided into blank group,model group,positive group,low-,medium-,and high-dose groups(750,1 500,3 000 mg·kg-1)of P.rockii pollen,and D-galactose(500 mg·kg-1)was injected subcu-taneously on the back of the neck to replicate the suba-cute aging model.Morris water maze was used to eval-uate the learning and memory of mice,HE and DAPI staining were used to evaluate the damage of liver and brain tissue cells,and ELISA was used to detect the levels of MDA,T-AOC,GSH-Px,SOD,CAT and γ-H2AX and SA-β-Gal in serum,liver and brain tis-sues.Results The water maze experiment showed that P.rockii pollen could significantly improve the learning and memory of aging mice.Tissue staining showed that P.rockii pollen had a protective effect on liver and brain tissue cell injury in D-galactose-induced aging mice,and the effect was most obvious in the high-dose group.ELISA results showed that compared with the model group,the levels of MDA,γ-H2AX and SA-β-Gal in each dose group of P.rockii pollen decreased,and the levels of T-AOC,GSH-Px,SOD and CAT increased.Conclusions P.rockii pollen can improve the learning and memory of D-galactose-induced aging model mice,improve the activity of an-tioxidant enzymes and the ability to scavenge free radi-cals and lipid peroxides in aging mice,and protect liv-er and brain tissue cells from oxidative damage,sug-gesting that it has a potential anti-aging effect.关键词
紫斑牡丹花粉/D-半乳糖/氧化应激/抗衰老/Morris水迷宫/HE染色/DAPI染色Key words
P.rockii pollen/D-galactose/oxidative stress/anti-aging/Morris water maze/HE staining/DAPI staining分类
医药卫生引用本文复制引用
彭腾腾,王信,李海燕,尹盼盼,范彬,石晓峰..紫斑牡丹花粉对D-半乳糖致衰老模型小鼠的影响[J].中国药理学通报,2024,40(4):730-739,10.基金项目
甘肃省兰州市人才创新项目(No 2021-RC-112) （No 2021-RC-112）
甘肃省兰州市科技计划项目(No 2020-XG-21) （No 2020-XG-21）
甘肃省科技计划资助项目(No 23CXGA0038) （No 23CXGA0038）
