中国药理学与毒理学杂志2024，Vol.38Issue(4)：249-255,7.DOI:10.3867/j.issn.1000-3002.2024.04.002
2,5-二甲氧基-4-碘苯丙胺对自由活动小鼠次级视觉皮质V2区γ振荡的作用
Effect of 2,5-dimethoxy-4-iodoamphetamine on γ oscillations in area V2 of secondary visual cortex in freely moving mice
王福旗 1田京伟 2张树卓 3苏瑞斌3
作者信息
- 1. 烟台大学药学院,山东烟台 264005||军事科学院军事医学研究院毒物药物研究所,国家安全特需药品全国重点实验室,神经精神药理学北京市重点实验室,北京 100850
- 2. 烟台大学药学院,山东烟台 264005
- 3. 军事科学院军事医学研究院毒物药物研究所,国家安全特需药品全国重点实验室,神经精神药理学北京市重点实验室,北京 100850
- 折叠
摘要
Abstract
OBJECTIVE To investigate the effect of the 5-hydroxytryptamine 2A receptor(5-HT2AR)agonist 2,5-dimethoxy-4-iodoamphetamine(DOI)on field potentials in area V2 of the secondary visual cortex in freely moving mice.METHODS C57BL/6J mice were embedded with electroencephalocorti-cal(ECOG)electrodes and recovered for 7 d after surgery.After 1%DMSO(vehicle)was ip given and recording of local field potentials(LFP)in area V2 of the secondary visual cortex for 30 min,DOI(2.0 mg·kg-1,ip)was administered and LFP in area V2 was recorded for 30 min before the 5-HT2AR antagonist volinanserin(0.3 mg·kg-1,ip)was administered and LFP in area V2 was recorded for 30 min.The recorded data were imported into Neuro Explorer software for spectral analysis.GraphPad Prism 8.0.2 software was used to statistically analyze the changes in power spectral density of γ and θ oscillations before and after administration while Matlab software was used to analyze the phase amplitude coupling of θ and γ oscillations.RESULTS Compared with the vehicle group,DOI(2.0 mg·kg-1,ip)significantly increased the power of γ oscillations(30-80 Hz)and θ oscillations(4-7 Hz)in area V2 of the secondary visual cortex(P＜0.05),and the modulation index of phase amplitude coupling between θ and γ oscilla-tions was significantly reduced(P＜0.01).The administration of volinanserin reversed the DOI-induced enhancement of the power of γ and θ oscillations in area V2 of the secondary visual cortex(P＜0.05).CONCLUSION DOI may exert its hallucinogenic effects by increasing the power of γ oscillations and θ oscillations in area V2 of the secondary visual cortex and by decreasing the phase amplitude coupling of θ oscillations to γ oscillations in mice.关键词
次级视觉皮质/γ振荡/皮质脑电/2,5-二甲氧基-4-碘苯丙胺/局部场电位Key words
secondary visual cortex/γ oscillations/cortical electroencephalography/2,5-dimethoxy-4-iodoamphetamine/local field potentials分类
医药卫生引用本文复制引用
王福旗,田京伟,张树卓,苏瑞斌..2,5-二甲氧基-4-碘苯丙胺对自由活动小鼠次级视觉皮质V2区γ振荡的作用[J].中国药理学与毒理学杂志,2024,38(4):249-255,7.
