2,5-二甲氧基-4-碘苯丙胺对自由活动小鼠次级视觉皮质V2区γ振荡的作用OA北大核心CSTPCD

OBJECTIVE To investigate the effect of the 5-hydroxytryptamine 2A receptor(5-HT2AR)agonist 2,5-dimethoxy-4-iodoamphetamine(DOI)on field potentials in area V2 of the secondary visual cortex in freely moving mice.METHODS C57BL/6J mice were embedded with electroencephalocorti-cal(ECOG)electrodes and recovered for 7 d after surgery.After 1%DMSO(vehicle)was ip given and recording of local field potentials(LFP)in area V2 of the secondary visual cortex for 30 min,DOI(2.0 mg·kg-1,ip)was administered and LFP in area V2 was recorded for 30 min before the 5-HT2AR antagonist volinanserin(0.3 mg·kg-1,ip)was administered and LFP in area V2 was recorded for 30 min.The recorded data were imported into Neuro Explorer software for spectral analysis.GraphPad Prism 8.0.2 software was used to statistically analyze the changes in power spectral density of γ and θ oscillations before and after administration while Matlab software was used to analyze the phase amplitude coupling of θ and γ oscillations.RESULTS Compared with the vehicle group,DOI(2.0 mg·kg-1,ip)significantly increased the power of γ oscillations(30-80 Hz)and θ oscillations(4-7 Hz)in area V2 of the secondary visual cortex(P＜0.05),and the modulation index of phase amplitude coupling between θ and γ oscilla-tions was significantly reduced(P＜0.01).The administration of volinanserin reversed the DOI-induced enhancement of the power of γ and θ oscillations in area V2 of the secondary visual cortex(P＜0.05).CONCLUSION DOI may exert its hallucinogenic effects by increasing the power of γ oscillations and θ oscillations in area V2 of the secondary visual cortex and by decreasing the phase amplitude coupling of θ oscillations to γ oscillations in mice.