结核分枝杆菌膜囊泡RNA表达谱分析及功能研究OA北大核心CSTPCD

Objective This study aims to depict the characteristic RNA expression profile of mycobacterium tuberculosis membrane vesicles(MVs),analyse their main biological functions,and preliminarily explore their role in inducing apoptosis of macrophages.Methods MVs of mycobacterium tuberculosis were isolated,total RNA was extracted,and double-end sequencing was performed using the illumina Hiseq 2500-SE50 platform.Sequence comparison was performed using BWA software,and the genes were functionally annotated by COG database.Cell viability was detected by MTT assay and apoptosis was detected by Annexin-V combined with PI staining.Results The present study depicted the types and content of RNAs in mycobacterium tuberculosis MVs by RNA sequencing and bioinformatic analysis.The functional annotation through COG database showed that the major functions of those RNAs enriched were including lipid transport metabolism,amino acid transport metabolism,energy production and conversion,secondary metabolite synthesis transport and metabolism,and transcription.Bioinformatic analysis showed that the major functions enriched included lipid transport metabolism,amino acid transport metabolism,energy production and conversion,secondary metabolite synthesis transport and metabolism,and transcription.Functional assays showed that mycobacterium tuberculosis MVs could reduce the survival of THP-1-derived macrophages and promote apoptosis.Conclusions Mycobacterium tuberculosis MVs are rich in short RNA fragments,which have the effect of inducing apoptosis.However,its specific mechanism still needs more in-depth and detailed study.