中国医学前沿杂志（电子版）2024，Vol.16Issue(3)：28-33,6.DOI:10.12037/YXQY.2024.03-06
结核分枝杆菌膜囊泡RNA表达谱分析及功能研究
RNA profiling and functional study of mycobacterium tuberculosis membrane vesicles
摘要
Abstract
Objective This study aims to depict the characteristic RNA expression profile of mycobacterium tuberculosis membrane vesicles(MVs),analyse their main biological functions,and preliminarily explore their role in inducing apoptosis of macrophages.Methods MVs of mycobacterium tuberculosis were isolated,total RNA was extracted,and double-end sequencing was performed using the illumina Hiseq 2500-SE50 platform.Sequence comparison was performed using BWA software,and the genes were functionally annotated by COG database.Cell viability was detected by MTT assay and apoptosis was detected by Annexin-V combined with PI staining.Results The present study depicted the types and content of RNAs in mycobacterium tuberculosis MVs by RNA sequencing and bioinformatic analysis.The functional annotation through COG database showed that the major functions of those RNAs enriched were including lipid transport metabolism,amino acid transport metabolism,energy production and conversion,secondary metabolite synthesis transport and metabolism,and transcription.Bioinformatic analysis showed that the major functions enriched included lipid transport metabolism,amino acid transport metabolism,energy production and conversion,secondary metabolite synthesis transport and metabolism,and transcription.Functional assays showed that mycobacterium tuberculosis MVs could reduce the survival of THP-1-derived macrophages and promote apoptosis.Conclusions Mycobacterium tuberculosis MVs are rich in short RNA fragments,which have the effect of inducing apoptosis.However,its specific mechanism still needs more in-depth and detailed study.关键词
结核分枝杆菌/膜囊泡/核糖核酸测序/细胞凋亡Key words
Mycobacterium tuberculosis/Membrane vesicles/RNA sequencing/Apoptosis引用本文复制引用
吕翎娜,刘秋月..结核分枝杆菌膜囊泡RNA表达谱分析及功能研究[J].中国医学前沿杂志（电子版）,2024,16(3):28-33,6.基金项目
National Natural Science Foundation of China(82372260,82100011),Beijing Natural Science Foundation Haidian Original Innovation Joint Fund(L202023) 国家自然科学基金(82372260,82100011) （82372260,82100011）
北京市自然科学基金海淀原始创新联合项目(L202023) （L202023）
