中国肿瘤生物治疗杂志2024，Vol.31Issue(4)：351-358,8.DOI:10.3872/j.issn.1007-385x.2024.04.005
牛蒡子苷元调控Notch/Hes-1信号通路对口腔鳞状细胞癌HSC-3细胞增殖、凋亡和侵袭的影响
Effects of arctigenin on the proliferation,apoptosis,and invasion of oral squamous cell carcinoma HSC-3 cells by regulating the Notch/Hes-1 signaling pathway
摘要
Abstract
Objective:To investigate the effects of arctigenin(ARC)on the proliferation,apoptosis and invasion of oral squamous cell carcinoma(OSCC)HSC-3 cells by regulating the Notch/Hes-1 signaling pathway and its mechanism.Methods:Human HSC-3 cells were treated with different mass concentrations of ARC,and the effect of ARC on cell proliferation was detected by CCK-8 method to select the appropriate drug concentration.HSC-3 cells were divided into the control group,the ARC-L group(10 mg/L ARC),the ARC-M group(20 mg/L ARC),the ARC-H group(40 mg/L ARC),and the ARC-H+Jagged1/FC group(40 mg/L ARC+1.2 μg/mL Jagged1/FC).Cell proliferation ability was detected by EdU assay,and cell migration and invasion abilities,cell cycle and apoptosis rate were detected by scratch healing assay,Transwell assay and flow cytometry,respectively.The expression levels of proliferation(c-Myc,cyclin D1),apoptosis(BAX,Bcl-2,survivin),EMT(E-cadherin,vimentin,Snail)and Notch/Hes-1 pathway(Notch1,Hes-1,NICD)related proteins were detected by Western blot.Results:Compared with 0 mg/L,10-80 mg/L of ARC significantly decreased the proliferation vitality of HSC-3 cells(all P<0.05).Compared with the control group,the EdU positive rate,scratch healing rate,invasion cell number,proportions of S phase and G2/M phase cells,and the expressions of c-Myc,cyclin D1,Bcl-2,survivin,vimentin,Snail,Notch 1,Hes-1,and NICD proteins of HSC-3 cells decreased significantly in the ARC-L,ARC-M,and ARC-H groups(all P<0.05);the apoptosis rate,the proportion of G0/G1 phase cells,and the expressions of BAX and E-cadherin proteins increased significantly(all P<0.05),and were concentration gradient dependent.Simultaneous use of Notch agonist Jagged1/FC partially reversed the effects of ARC on the proliferation,migration,invasion,apoptosis and the expressions of related proteins of HSC-3 cells(all P<0.05).Conclusion:ARC may inhibit the proliferation and invasion of OSCC HSC-3 cells and promote cell apoptosis by inhibiting the Notch/Hes-1 signaling pathway.关键词
牛蒡子苷元/口腔鳞状细胞癌/HSC-3细胞/增殖/凋亡/侵袭/Notch/Hes-1信号通路Key words
arctigenin(ARC)/oral squamous cell carcinoma(OSCC)/HSC-3 cell/proliferation/apoptosis/invasion/Notch/Hes-1 signal pathway分类
医药卫生引用本文复制引用
任丽洁,刘孟媛,史冠忠,唐亮..牛蒡子苷元调控Notch/Hes-1信号通路对口腔鳞状细胞癌HSC-3细胞增殖、凋亡和侵袭的影响[J].中国肿瘤生物治疗杂志,2024,31(4):351-358,8.基金项目
河北省卫健委2022年度医学科学研究课题(No.20220373) （No.20220373）
