中国肿瘤生物治疗杂志2024，Vol.31Issue(4)：365-370,6.DOI:10.3872/j.issn.1007-385x.2024.04.007
桃叶珊瑚苷调控RhoA/ROCK信号通路对胃癌MGC803细胞上皮间质转化和血管生成拟态的影响
Effects of aucubin on epithelial mesenchymal transition and vasculogenic mimicry in gastric cancer MGC803 cells by regulating the RhoA/ROCK signaling pathway
摘要
Abstract
Objective:To investigate the effects of aucubin(AU)on epithelial interstitial transformation(EMT)and vasculogenic mimicry(VM)formation in gastric cancer MGC803 cells by regulating the RhoA/ROCK signaling pathway.Methods:Human gastric cancer MGC803 cells were routinely cultured and randomly divided into the Control group,the AU-L group(20 μmol/L AU),the AU-M group(40 μmol/L AU),the AU-H group(80 μmol/L AU),and the AU-H+RhoA activator naciclassine(Nar)group(AU-H+Nar group,80 μmol/L AU+30 μmol/L Nar).The effects of AU on cell proliferation,invasion and migration were detected by CCK-8 assay,Transwell assay and cell scratch assay,respectively.Three-dimensional cell culture was applied to observe the effects of different concentration of AU on the formation of VM lumen in vitro in each group.The effects of different concentration of AU on the expressions of RhoA,ROCK,VM,and EMT related proteins in each group were detected by Western blot.Results:Compared with those in the Control group,the proliferation rates(48 and 72 h),the cell migration rate,the number of cell invasions,the number of VM lumen structures,the protein expressions of RhoA,ROCK1,N-cadherin,vimentin,and VE cadherin in MGC803 cells in the AU-M and AU-H groups reduced significantly(all P<0.05).The expression of E-cadherin increased significantly(P<0.05).Simultaneous use of Nar significantly weakened the inhibitory effect of AU on EMT and VM formation in MGC803 cells(all P<0.05).Conclusion:AU inhibits the proliferation,migration,invasion,EMT and VM formation processes of gastric cancer MGC803 cells by down-regulating the RhoA/ROCK signaling pathway.关键词
桃叶珊瑚苷/RhoA/ROCK信号通路/胃癌/MGC803细胞/上皮间质转化/血管生成拟态Key words
aucubin(AU)/RhoA/ROCK signaling pathway/gastric cancer/MGC803 cell/epithelial interstitial transformation(EMT)/vasculogenic mimicry(AM)分类
医药卫生引用本文复制引用
宋鸣,周昕,刘俊,胡伟,张敏,郑新..桃叶珊瑚苷调控RhoA/ROCK信号通路对胃癌MGC803细胞上皮间质转化和血管生成拟态的影响[J].中国肿瘤生物治疗杂志,2024,31(4):365-370,6.基金项目
湖北省中医药管理局中医药科研项目(No.ZY2023F061) （No.ZY2023F061）
