中国肿瘤生物治疗杂志2024，Vol.31Issue(4)：371-376,6.DOI:10.3872/j.issn.1007-385x.2024.04.008
PD-1单抗联合顺铂或吉西他滨化疗对KRAS突变非小细胞肺癌A549细胞移植瘤小鼠模型的治疗作用
Therapeutic effect of PD-1 monoclonal antibody combined with cisplatin or gemcitabine chemotherapy in a mouse xenograft model with KRAS mutant non-small cell lung cancer A549 cells
摘要
Abstract
Objective:To investigate the therapeutic effect of programmed death-1 monoclonal antibody(PD-1 mAb)combined with cisplatin and gemcitabine in the treatment of transplanted tumor with KRAS gene mutant non-small cell lung cancer(NSCLC)A549 cells in mice.Methods:By constructing an immune system-tumor humanized mouse xenograft model with NSCLC A549 cells,60 mice were randomly divided into 6 groups(10 mice/group),namely control group(200 μL/kg PBS),PD-1 mAb group(20 mg/kg PD-1 mAb),cisplatin group(3 mg/kg cisplatin),PD-1 mAb+cisplatin group(20 mg/kg PD-1 mAb+3 mg/kg cisplatin),gemcitabine group(30 mg/kg gemcitabine)and PD-1 mAb+gemcitabine group(20 mg/kg PD-1 mAb+30 mg/kg gemcitabine).TUNEL and DAPI double staining were used to detect the level of apoptosis in transplanted tumor tissues.The volume and mass of transplanted tumors were detected,and the growth inhibition rate of transplanted tumor was measured.The microvessel density(MVD)of transplanted tumor was determined by immunohistochemistry.Results:The humanized mouse xenograft model of NSCLC A549 cells was successfully constructed.Compared with the other five groups,the apoptosis rate and tumor growth inhibition rate in the PD-1 mAb+cisplatin group were the highest,and the tumor volume,mass,and MVD were the smallest(all P<0.05).Conclusion:Cisplatin has a synergistic activity with PD-1 mAb,while gemcitabine can antagonize the therapeutic effect of PD-1 mAb.It is suggested that PD-1 mAb combined with cisplatin chemotherapy is better for KRAS mutant NSCLC A549 cell transplanted tumor mice.关键词
PD-1单抗/顺铂/吉西他滨/非小细胞肺癌/KRAS突变/免疫治疗Key words
PD-1 monoclonal antibody(PD-1 mAb)/cisplatin/guicitabine/non-small cell lung cancer(NSCLC)/KRAS mutation/immunotherapy分类
医药卫生引用本文复制引用
李雄兵,周瑞芬,李佳丽,王汉姣,王超,李婧,曹喆,舒诚荣..PD-1单抗联合顺铂或吉西他滨化疗对KRAS突变非小细胞肺癌A549细胞移植瘤小鼠模型的治疗作用[J].中国肿瘤生物治疗杂志,2024,31(4):371-376,6.基金项目
湖北省卫生健康委员会科研项目(No.WJ2019M095) （No.WJ2019M095）
