中成药2024，Vol.46Issue(4)：1093-1102,10.DOI:10.3969/j.issn.1001-1528.2024.04.006
UPLC-Q-TOF-MS/MS法分析紫辛鼻鼽颗粒化学成分及入血成分
Analyses of chemical constituents and constituents absorbed into blood in Zixin Biqiu Granules
摘要
Abstract
AIM To establish a UPLC-Q-TOF-MS/MS method for the analyses of chemical constituents and constituents absorbed into blood in Zixin Biqiu Granules.METHODS The analysis was performed on a 30℃thermostatic Agilent ZORBAX SB-C18 column(1.8 μm,2.1 mm×100 mm),with the mobile phase comprising of 0.2%formic acid-acetonitrile flowing at 0.2 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode,after which constituent identification was employed according to excimer ions,retention time,secondary fragment ions combined with reference substance information,relevant literatures.RESULTS Total 88 constituents were identified,28 of which were validated by comparison of reference substances.Thirty constituents were observable in rat serum,20 of which were prototype constituents,10 of which were metabolites.CONCLUSION This stable and reliable method can lay the foundation for research on pharmacological substance basis and quality control of Zixin Biqiu Granules.关键词
紫辛鼻鼽颗粒/化学成分/入血成分/UPLC-Q-TOF-MS/MSKey words
Zixin Biqiu Granules/chemical constituents/constituents absorbed into blood/UPLC-Q-TOF-MS/MS分类
医药卫生引用本文复制引用
康雨,胡军华,章晨峰,肖伟,戴翠红,林夏,王振中..UPLC-Q-TOF-MS/MS法分析紫辛鼻鼽颗粒化学成分及入血成分[J].中成药,2024,46(4):1093-1102,10.基金项目
国家中医药管理局岐黄学者项目(2021) （2021）
