中成药2024，Vol.46Issue(4)：1186-1191,6.DOI:10.3969/j.issn.1001-1528.2024.04.019
乌头根际土壤钩状木霉菌次生代谢产物化学成分研究
Chemical constituents from the secondary metabolites of Trichoderma hamatum in rhizosphere soil of Aconitum carmichaelii
摘要
Abstract
AIM To study the chemical constituents from secondary metabolites of Trichoderma hamatum in rhizosphere soil of Aconitum carmichaelii Debx.METHODS Based on the strategy of chemical epigenetic modification,the secondary metabolites of T.hamatum were obtained by culturing in fungus No.2 medium.Compounds were separated and purified from T.hamatum ethyl acetate extract by reversed-phase column chromatography,dynamic axial compression chromatography,C18 column chromatography,semi-preparative HPLC.The structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Twelve compounds were isolated and identified as 4-acetyl-3-hydroxy-6-methy-pyran-2-one(1),3-methyl-4-phenyl-2(5H)-furanone(2),6-pentyl-α-pyrone(3),volemolide(4),lignoren(5),enniatin A1(6),wickerols A(7),demethylincisterol A3(8),(22E)-5a,8a-epidioxyergosta-6,22-dien-3b-ol(9),daidzein(10),cyclo(L)-Pro-(L)-Phe(11),Cyclo(D)-Pro-(D)-Ile(12).CONCLUSION Compounds 1-12 are obtained from T.hamatum for the first time,and compound 1 is found from Trichoderma genus for the first time.关键词
钩状木霉菌/次生代谢产物/乌头/根际土壤/化学成分/分离鉴定Key words
Trichoderma hamatum/secondary metabolites/Aconitum carmichaelii Debx/rhizosphere soil/chemical constituents/isolation and identification分类
中医学引用本文复制引用
刘然,黄利,刘梦丹,唐毓隆,郭大乐,王光志..乌头根际土壤钩状木霉菌次生代谢产物化学成分研究[J].中成药,2024,46(4):1186-1191,6.基金项目
四川省科技厅基础研究计划项目(2021YFN0134,2021YJ0110) （2021YFN0134,2021YJ0110）
