中南民族大学学报（自然科学版）2024，Vol.43Issue(3)：319-325,7.DOI:10.20056/j.cnki.ZNMDZK.20240304
基于果糖和多巴胺的碳点制备及性能
Preparation and properties of carbon dots based on fructose and dopamine
摘要
Abstract
Nitrogen doped carbon dots(N-CDs)were synthesized via one-step hydrothermal method using fructose as carbon source and dopamine(DA)as nitrogen source.The structure,morphology,surface functional groups and optical properties of N-CDs were characterized via X-ray diffraction(XRD),high-resolution transmission electron microscopy(HRTEM),X-ray photoelectron spectrometer(XPS),ultraviolet-visible absorption spectrum(UV-vis),Fourier-transform infrared spectroscopy(FT-IR)and other characterization techniques.The cytotoxicity was studied by MTT assay,and cell imaging performance was studied by confocal microscopy.The results showed that the N-CDs were spherical particles with an average particle size of about 1.76 nm.The surface was rich in functional groups,such as hydroxyl,amino,carboxyl and carbonyl groups.The fluorescence excitation and emission wavelengths of the N-CDs were 440 nm and 536 nm,respectively.The N-CDs demonstrated good water solubility and low cytotoxicity.At a concentration of 0.5 mg∙mL-1,the survival rate of Hela cells was higher than 93%.N-CDs could easily enter the cytoplasm through the cell membrane and showed bright fluorescence signal in the cell.This method is simple,green and environmentally friendly.The N-CDs have low cytotoxicity and can be used in intracellular fluorescence imaging,which is highly desirable for the early diagnosis of cancer cells.关键词
果糖/多巴胺/碳点/荧光性能/细胞成像Key words
fructose/dopamine/carbon dots/fluorescence properties/cell imaging分类
化学化工引用本文复制引用
张洋洋,崔丽丽,刘科,程盈盈,余燕敏..基于果糖和多巴胺的碳点制备及性能[J].中南民族大学学报（自然科学版）,2024,43(3):319-325,7.基金项目
国家自然科学基金资助项目(51809087) （51809087）
中国国家留学基金资助项目(202008420051) （202008420051）
湖北省教育厅科学技术研究资助项目(Q20182601) （Q20182601）
