作物学报2024，Vol.50Issue(5)：1091-1103,13.DOI:10.3724/SP.J.1006.2024.34142
大豆根茎过渡区弯曲突变体Mrstz的鉴定与基因定位
Identification and gene mapping of soybean mutant Mrstz in root-stem transition zone
摘要
Abstract
Root-stem transition zone(RSTZ)connects roots and stems,and its morphology modifies the structure of aerial part and lodging resistance potential in soybean.In this study,a soybean mutant Mrstz,appearing with curved or rotated RSTZ,was obtained by EMS mutagenesis.Its morphological characteristics were stably inherited and could provide specific sources for exploring the development patterns of soybean stems.Therefore,this mutant was crossed with Zhonghuang 13 to construct a recombinant inbred line population.By comparing the anatomical structure of vertical RSTZ and curved RSTZ,the wider vascular cambium,more secondary xylem cell layers and irregular cell shape were identified in curved lines,indicating that vas-cular cambium differentiation may be one of the important factors leading to the difference of RSTZ morphology.Subsequently,the chemical compositions of lines with vertical RSTZ or curved RSTZ were determined,respectively.It was found that the higher lignin and crude fiber content in the RSTZ,the more difficult to bend.The RSTZ with curved or vertical lines were further selected for BSA-seq.SNP-index and InDel-index methods were employed to mine a significant association region,Chr.19:43,030,943-45,849,854 containing 319 genes,which may regulate RSTZ morphology.Combined bioinformatics analysis,gene annotation information and expression abundance analysis,seven candidate genes(Glyma.19G170200,Glyma.19G201500,Glyma.19G187800,Glyma.19G178200,Glyma.19G197000,Glyma.19G179100,Glyma.19G196900)were screened.Among them,Glyma.19G187800,Glyma.19G178200,and Glyma.19G196900 potentially affected RSTZ morphogenesis in soybean domestica-tion.This study not only provides germplasm resources for the understanding of soybean RSTZ tissue formation and its genetic basis,but also provides new insights for further exploration of genes regulating soybean stalk development.关键词
大豆/根茎过渡区/突变体/BSA-seq/候选基因Key words
soybean/root and stem transition zone/mutant/BSA-seq/candidate genes引用本文复制引用
苗龙,王晓波,邱丽娟,舒阔,李娟,黄茹,王业杏,Soltani Muhammad YOUSOF,许竞好,吴传磊,李佳佳..大豆根茎过渡区弯曲突变体Mrstz的鉴定与基因定位[J].作物学报,2024,50(5):1091-1103,13.基金项目
本研究由国家重点研发计划项目(2021YFD1201605),安徽省自然科学基金项目(2308085MC88,2108085QC114)和安徽农业大学自然科学基金项目(k2031005)资助.This study was supported by the National Key Research and Development Program of China(2021YFD1201605),the Natural Science Foun-dation of Anhui Province(2308085MC88,2108085QC114),and the Natural Science Foundation Project of Anhui Agricultural University(k2031005). （2021YFD1201605）
