作物学报2024，Vol.50Issue(5)：1104-1114,11.DOI:10.3724/SP.J.1006.2024.32043
水稻雄性不育突变体tpa1的表型鉴定与精细定位
Phenotypic identification and fine mapping of male sterile mutant tpa1 in rice
摘要
Abstract
Male sterile material is the key to hybrid rice breeding.In this study,a male sterile mutant tpa1 was screened by ethyl methane sulfonate(EMS)mutagenesis of excellent indica cultivar Xinong 1B.There was no difference between tpa1 and wild type at vegetative growth stage.At reproductive stage,male gametes were sterile and female gametes developed normally.Phenotypic observation showed that the pollen of tpa1 was completely broken and disappeared,the stratum corneum of the outer wall of the anther was abnormal,the Ubisch body of the inner wall of the anther was abnormal,the callose synthesis was abnormal,the tapetum apoptosis was abnormal,and the outer wall of the pollen was missing bacula layer.Genetic analysis showed that the mutant trait was controlled by a pair of recessive nuclear genes.A genetic population was constructed using the tpa1 mutant and Jinhui 10.Finally,TPA1 gene was located between the markers N9 and N11 on chromosome 4,with a physical distance of 74 kb.There were 15 predicted genes in this interval.Resequencing identified that only a single base sub-stitution occurred in the exon of LOC_Os04g53380,leading to an early termination of translation.Subsequent sequencing of the wild-type and mutant tpa1 confirmed this mutation,thus identified the gene as a candidate gene for TPA1,indicating that TPA1 was a new male sterile gene.This study will lay a foundation for the functional study of TPA1 gene.关键词
水稻(Oryza sativa L.)/雄性隐性核不育/tpa1/精细定位Key words
rice(Oryza sativa L.)/male recessive nuclear infertility/tpa1/fine mapping引用本文复制引用
万应春,班义结,蒋钰东,王亚欣,刘晶晶,刘晓晴,程育林,王楠,冯萍..水稻雄性不育突变体tpa1的表型鉴定与精细定位[J].作物学报,2024,50(5):1104-1114,11.基金项目
本研究由重庆市研究生科研创新项目(CYS22224)和重庆市技术创新与应用发展专项重点项目(CSTB2022TIAD-KPX0014)资助.This study was supported by the Chongqing Graduate Research and Innovation Project(CYS22224)and the Chongqing Technology Innova-tion and Application Development Special Key Project(CSTB2022TIAD-KPX0014). （CYS22224）
