水稻雄性不育突变体tpa1的表型鉴定与精细定位OA北大核心CSTPCD

Male sterile material is the key to hybrid rice breeding.In this study,a male sterile mutant tpa1 was screened by ethyl methane sulfonate(EMS)mutagenesis of excellent indica cultivar Xinong 1B.There was no difference between tpa1 and wild type at vegetative growth stage.At reproductive stage,male gametes were sterile and female gametes developed normally.Phenotypic observation showed that the pollen of tpa1 was completely broken and disappeared,the stratum corneum of the outer wall of the anther was abnormal,the Ubisch body of the inner wall of the anther was abnormal,the callose synthesis was abnormal,the tapetum apoptosis was abnormal,and the outer wall of the pollen was missing bacula layer.Genetic analysis showed that the mutant trait was controlled by a pair of recessive nuclear genes.A genetic population was constructed using the tpa1 mutant and Jinhui 10.Finally,TPA1 gene was located between the markers N9 and N11 on chromosome 4,with a physical distance of 74 kb.There were 15 predicted genes in this interval.Resequencing identified that only a single base sub-stitution occurred in the exon of LOC_Os04g53380,leading to an early termination of translation.Subsequent sequencing of the wild-type and mutant tpa1 confirmed this mutation,thus identified the gene as a candidate gene for TPA1,indicating that TPA1 was a new male sterile gene.This study will lay a foundation for the functional study of TPA1 gene.