作物学报2024，Vol.50Issue(5)：1136-1146,11.DOI:10.3724/SP.J.1006.2024.34152
转录组结合区域关联分析挖掘油菜含油量积累的候选基因
A combination of genome-wide association and transcriptome analysis reveal candidate genes affecting seed oil accumulation in Brassica napus
摘要
Abstract
Rapeseed(Brassica napus L.)is the main source of edible vegetable oil in China,and increasing seed oil content is the most effective way to increase the supply of rapeseed oil.In this study,43 genes related to lipid synthesis were selected by ana-lyzing the seed transcriptome data of 4 rapeseed inbred lines 25,35,and 45 days after pollination.Among them,33 genes were continuously up-expressed and 10 genes were continuously down-expressed from 25 to 45 days of seed development.The main genes included BnLEC1,BnABI5,BnOLEO4,and BnOBAP1a.At the same time,combined with the resequencing data of 50 semi-winter Brassica napus,3 SNPs and 9 SNPs significantly related to oil content were detected to BnOBAP1a-A10 and BnABI5-A05,respectively,and the oil content of BnOBAP1A-A10_Hap1 was significantly higher than Hap2.The oil content of BnABI5-A05_Hap1 was significantly higher than Hap3.In addition,WGCNA was used to construct gene networks,and we found that BnOBAP1a and BnABI5 were indirectly connected through three transcription factors LEC1,HMGB3,and HTA11,which together formed a molecular network involved in the potential regulation of seed oil accumulation.The results of this study pro-vide valuable insights for the development of haplotype functional markers,aiming to further enhance oil content in B.napus.关键词
甘蓝型油菜/含油量/转录组分析/共表达分析/区域关联分析Key words
Brassica napus/oil content/transcriptome analysis/coexpression analysis/regional association analysis引用本文复制引用
曹松,钱论文,熊兴华,姚敏,任睿,贾元,向星汝,李文,何昕,刘忠松,官春云..转录组结合区域关联分析挖掘油菜含油量积累的候选基因[J].作物学报,2024,50(5):1136-1146,11.基金项目
本研究由湖南省杰出青年科学基金项目(2022JJ10027),湖南省教育厅科学研究重点项目(21A0135)和国家科技重大专项项目(2022ZD04009)资助.This study was supported by the Science Foundation for Distinguished Youth Scholars of Hunan Province,China(2022JJ10027),the Research Foundation of Education Bureau of Hunan Province,China(21A0135),and the National Key Science and Technology Project(2022ZD04009). （2022JJ10027）
