作物学报2024，Vol.50Issue(5)：1147-1157,11.DOI:10.3724/SP.J.1006.2024.34148
棉花种质资源萌发期耐盐性鉴定及筛选
Identification and screening of salt tolerance of cotton germplasm resources at germination stage
摘要
Abstract
Seed germination stage is sensitive to salt.The determination of cotton seed germination under different salt concentra-tion stress is one of the important bases for screening cotton salt-tolerant germplasm.In this study,629 cotton germplasm re-sources were treated with 0 mmol L-1 and 150 mmol L-1 NaCl,and the salt tolerance coefficients of 6 traits such as fresh weight and germination potential were analyzed.The results showed that there were significant differences in each trait under salt stress compared with the control.Principal component analysis and membership function analysis were used to comprehensively evalu-ate the salt tolerance of cotton germplasm.Cluster analysis was carried out on the comprehensive evaluation value(D-value),and 629 germplasm resources were divided into 5 categories according to the D-value:188 medium salt-tolerant materials,376 salt-tolerant materials,36 high salt-tolerant materials,28 salt-sensitive materials,1 high salt-sensitive material.A prediction model for salt tolerance evaluation of cotton at germination stage was established by stepwise regression analysis:D = 0.277RFW +0.29RGP + 0.189RPL + 0.387RGR-0.32(R2 =0.992).Four indexes of fresh weight,hypocotyl length,germination potential,and germination rate were selected as the indexes of salt tolerance identification in cotton germination stage.In this study,a set of accurate and efficient salt tolerance identification system was established,and 4 high salt-tolerant materials and 1 high salt-sensitive material were screened,which providing the reference for the study of salt tolerance mechanism and the cultivation of new salt tolerant varieties in cotton.关键词
棉花/萌发期/耐盐性/综合评价Key words
cotton/germination stage/salt tolerance/comprehensive evaluation引用本文复制引用
李航,余渝,刘丽,黄乾,刘文豪,司爱君,孔宪辉,王旭文,赵福相,梅拥军..棉花种质资源萌发期耐盐性鉴定及筛选[J].作物学报,2024,50(5):1147-1157,11.基金项目
本研究由新疆生产建设兵团第三师重大科技计划项目(KY2022ZD02),新疆生产建设兵团第三师科技创新人才项目(KJ2023PT01)和棉花生物学国家重点实验室开放课题(CB2022A27)资助.This study was supported by the Major science and Technology Plan Project of the Third Division of the Xinjiang Production and Construc-tion Corps(KY2022ZD02),the Science and Technology Innovation Talent Project of the Third Division of Xinjiang Production and Con-struction Corps(KJ2023PT01),and the State Key Laboratory of Cotton Biology(CB2022A27). （KY2022ZD02）
