棉花种质资源萌发期耐盐性鉴定及筛选OA北大核心CSTPCD

Seed germination stage is sensitive to salt.The determination of cotton seed germination under different salt concentra-tion stress is one of the important bases for screening cotton salt-tolerant germplasm.In this study,629 cotton germplasm re-sources were treated with 0 mmol L-1 and 150 mmol L-1 NaCl,and the salt tolerance coefficients of 6 traits such as fresh weight and germination potential were analyzed.The results showed that there were significant differences in each trait under salt stress compared with the control.Principal component analysis and membership function analysis were used to comprehensively evalu-ate the salt tolerance of cotton germplasm.Cluster analysis was carried out on the comprehensive evaluation value(D-value),and 629 germplasm resources were divided into 5 categories according to the D-value:188 medium salt-tolerant materials,376 salt-tolerant materials,36 high salt-tolerant materials,28 salt-sensitive materials,1 high salt-sensitive material.A prediction model for salt tolerance evaluation of cotton at germination stage was established by stepwise regression analysis:D = 0.277RFW +0.29RGP + 0.189RPL + 0.387RGR-0.32(R2 =0.992).Four indexes of fresh weight,hypocotyl length,germination potential,and germination rate were selected as the indexes of salt tolerance identification in cotton germination stage.In this study,a set of accurate and efficient salt tolerance identification system was established,and 4 high salt-tolerant materials and 1 high salt-sensitive material were screened,which providing the reference for the study of salt tolerance mechanism and the cultivation of new salt tolerant varieties in cotton.