作物学报2024，Vol.50Issue(5)：1172-1180,9.DOI:10.3724/SP.J.1006.2024.34167
陆地棉重组自交系再生能力和遗传转化效率筛选
Screening of regeneration capacity and genetic transformation efficiency in recombinant inbred lines of Gossypium hirsutum L.
摘要
Abstract
Transgenic engineered breeding is an effective way of cotton germplasm innovations.In order to expand the renewable genotypes of upland cotton and to enrich cotton transgenic receptors,we constructed an F9 recombinant inbred population(YE)by the single-seed descent method using the high-yielding broad leaf cotton variety Emian 22(E22)from Hubei province as the male parent and the high regeneration-capable okra leaf variety Yuzao 1(YZ1)as the female parent.We compared the callus induction rate(CIF),callus secondary fecundity(CSC),embryo rate of callus(CRE),and callus embryo time(CET)of 164 recombinant inbred lines using two different hormone combinations of IBA+KT(IK)and 2,4-D+KT(DK),respectively,which were mature tissue culture systems in cotton.A total of 12 regenerable broad leaf lines were obtained and evaluated for the genetic transforma-tion efficiency using the current mature genetic transformation system in cotton;meanwhile,agronomic traits of these lines were investigated.Finally,a family line YE3,with a genetic transformation efficiency of 82.9%and better agronomic traits,was ob-tained.This study develops a new genotype of upland cotton,which will facilitate the genetic transformation and gene function research of cotton.关键词
棉花/重组自交系/体细胞胚胎发生/再生能力/遗传转化效率Key words
cotton/RILs/somatic embryogenesis/regeneration capacity/transformation efficiency引用本文复制引用
乐愉,王涛,张献龙,林忠旭..陆地棉重组自交系再生能力和遗传转化效率筛选[J].作物学报,2024,50(5):1172-1180,9.基金项目
本研究由国家自然科学基金项目(32172025)资助.This study was supported by the National Natural Science Foundation of China(32172025). （32172025）
