陆地棉重组自交系再生能力和遗传转化效率筛选OA北大核心CSTPCD

Transgenic engineered breeding is an effective way of cotton germplasm innovations.In order to expand the renewable genotypes of upland cotton and to enrich cotton transgenic receptors,we constructed an F9 recombinant inbred population(YE)by the single-seed descent method using the high-yielding broad leaf cotton variety Emian 22(E22)from Hubei province as the male parent and the high regeneration-capable okra leaf variety Yuzao 1(YZ1)as the female parent.We compared the callus induction rate(CIF),callus secondary fecundity(CSC),embryo rate of callus(CRE),and callus embryo time(CET)of 164 recombinant inbred lines using two different hormone combinations of IBA+KT(IK)and 2,4-D+KT(DK),respectively,which were mature tissue culture systems in cotton.A total of 12 regenerable broad leaf lines were obtained and evaluated for the genetic transforma-tion efficiency using the current mature genetic transformation system in cotton;meanwhile,agronomic traits of these lines were investigated.Finally,a family line YE3,with a genetic transformation efficiency of 82.9%and better agronomic traits,was ob-tained.This study develops a new genotype of upland cotton,which will facilitate the genetic transformation and gene function research of cotton.