东北春播区糜子核心种质及其DNA分子身份证构建
Core germplasm and DNA molecular identity card of proso millet in Northeast Spring sowing region in China
摘要
Abstract
In this study,500 proso millet resources in Northeast Spring Sowing Region were used as the experimental materials,169 SSR markers,UPGMA clustering,and stratified sampling were used to construct core germplasm,and ID Analysis 4.0 soft-ware was used to construct molecular identity card.The genetic diversity of the core collection was evaluated by genetic diversity metrics such as allele number(Na),and the core collection was analyzed by PCOA.The results showed that 169 pairs of SSR primers were screened,and 30 pairs of SSR primers were found to have good polymorphism.The core collection of proso millet constructed by 30 pairs of SSR primers contained 190 materials,accounting for 38%of all germplasm.Ninety-one alleles were detected in all germplasm and core collection,and 100%alleles were retained.The number of effective alleles was 2.2977-2.9975 and 2.2872-3.0173,with an average of 2.8198 and 2.8297,respectively.The Shannon diversity index was 0.9532-1.0990 and 0.9535-1.1162,with an average of 1.0645 and 1.0667.The observed heterozygosity was 0.3434-0.8037 and 0.3162-0.7849,with an average of 0.5399 and 0.5359.The expected heterozygosity was 0.5654-0.6672 and 0.5645-0.6707,with an average of 0.6448 and 0.6473.Nei's gene diversity index was 0.5648-0.6664 and 0.5628-0.6686,with an average of 0.6441 and 0.6452.The poly-morphism information content was 0.6657-0.8356 and 0.6493-0.8340,with an average of 0.7974 and 0.7944.The results of t-test showed that there was no significant difference in the related indexes of molecular markers between all germplasm and core germplasm,and PCOA analysis showed that the core germplasm and all germplasm had similar genetic diversity and population structure.At the same time,8 SSR markers(RYW5,RYW8,RYW16,RYW28,RYW40,RYW53,RYW62,and RYW67)were found to distinguish 190 core germplasms,and the molecular identity card of the core germplasms of Northeast proso millet was constructed,which providing a scientific basis for the efficient utilization and rapid traceability of proso millet germplasm.关键词
糜子/东北春播区/SSR/核心种质/DNA分子身份证Key words
Panicum miliaceum/the Northeast Spring sowing region/SSR/core collection/DNA molecular ID card引用本文复制引用
丁艺冰,辛旭霞,冯智尊,曹越,郭娟,Dipak K SANTRA,王瑞云,陈喜明..东北春播区糜子核心种质及其DNA分子身份证构建[J].作物学报,2024,50(5):1181-1192,12.基金项目
本研究由山西农业大学杂粮种质创新与分子育种国家实验室(筹)(202204010910001)项目,国家自然科学基金项目(31271791),财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-06-14.5-A16),山西省现代农业产业技术体系建设专项资金项目(2023CYJSTX03-12),山西省重点研发项目(2022ZDYF110),山西农业大学杂粮种质创新与分子育种山西省重点实验室项目(K462202040),山西农业大学生物育种工程项目(YZGC069),山西农业大学农学院研究生教育改革项目(2023YJG05)和山西省科技成果转化引导专项(201904D131056)资助.This study was supported by the National Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding(in Prepare,202204010910001),the National Natural Science Foundation of China(31271791),the China Agriculture Research System of MOF and MARA(CARS-06-14.5-A16),the Special Fund for Construction of Modern Agricultural Industrial Technology System of Shanxi Province(2023CYJSTX03-12),the Key Research&Development Project of Shanxi Province(2022ZDYF110),the Shanxi Key Laboratory of Innovation and Molecular Breeding,Shanxi Agricultural University(K462202040),the Biological Breeding Project of Shanxi Agricultural University(YZGC069),and the Graduate Education Reform Project of Agronomy College in Shanxi Agricultural University(2023YJG05),and the Shanxi Province Scientific and Technological Achievements Transformation and Guidance Project(201904D131056). （筹）
