作物学报2024，Vol.50Issue(5)：1223-1235,13.DOI:10.3724/SP.J.1006.2024.34202
大豆籽粒Ve含量的全基因组关联分析
Genome-wide association study for vitamin E content in soybean(Glycine max L.)seed
摘要
Abstract
Vitamin E(Ve)is a natural antioxidant in soybean oil and an important index to evaluate the nutritional value of soy-bean oil.In this study,α-,γ-,and δ-contents of seed were determined from a natural soybean population containing 264 germ-plasm resources in 2021 and 2022,and genome-wide association study(GWAS)was conducted.A total of 199 SNPs significantly associated with soybean Ve content were detected in this study,among which 9 SNPs that could be repeatedly detected in two different environments or two traits,which located on chromosomes 3,7,11,12,13,15,17,and 18,respectively.Among them,the significant association signal on chromosome 7 was a major effect site controlling α-tocopherol content,which can be detected in two environments,with the explanation rate of phenotypic variation 9.83%.By screening the candidate genes of this site,Glyma.07G054000 encoding myb transcription factor was obtained,which may be the effect gene of this site.In addition,two genes encoding γ-tocopherol methyltransferase Glyma.12G014200 and Glyma.12G014300 obtained on chromosome 12,may be important genes affecting Ve content.The results of this study are helpful to analyze the genetic basis and regulatory mechanism of Ve content in soybean seed,and lay a foundation for genetic improvement of soybean quality.关键词
大豆/籽粒/Ve含量/全基因组关联分析/候选基因Key words
soybean/seed/Ve content/GWAS/candidate gene引用本文复制引用
张红梅,张威,王琼,贾倩茹,孟珊,熊雅文,刘晓庆,陈新,陈华涛..大豆籽粒Ve含量的全基因组关联分析[J].作物学报,2024,50(5):1223-1235,13.基金项目
本研究由江苏省农业自主创新基金项目(CX(22)5002)和国家自然科学基金项目(32001455,30771360)资助.This study was supported by the Jiangsu Agricultural Science and Technology Innovation Fund(CX(22)5002)and the National Natural Science Foundation of China(32001455,30771360). （CX(22）
