作物学报2024，Vol.50Issue(5)：1351-1360,10.DOI:10.3724/SP.J.1006.2024.32027
水稻OsCNGC10基因抗倒伏性以及抗旱性功能研究
Mechanism of loding residence and drought tolerance of OsCNGC10 gene in rice
摘要
Abstract
Cyclic nucleotide-gated ion channels are ligand-gated cationic channels that exist in animals and plants,which are an important part of eukaryotic signaling cascades.In this study,OsCNGC10(cyclic nucleotide-gated channel)gene in rice was used,and the overexpression vector pU1301-CNGC10-Flag and the double-target knockout vector pRGEB32-CRISPR/cas9-cngc10 were constructed.The knockout and overexpression materials were obtained by Agrobacterium-mediated genetic transformation.Homozygous plants oscngc10-2 and OE-CNGC10-6 were isolated from T2 generation.The analysis of stem characteristics and lodging resistance of transgenic plants showed that oscngc10-2 had enhanced stem strength and lodging resistance.Stem cell wall sections and tissue composition analysis showed that oscngc10-2 increased lodging resistance due to the increase of stem wall thickness,parenchyma cell abundance,and lignin content.The knockout of OsCNGC10 increased the lignin content and the abundance of stem-cell wall parenchyma cells.The overexpression of OsCNGC10 reduced stem wall thickness,lignin content,and cell abundance in stem cell wall,while the knockdown of OsCNGC10 increased lignin content and increased the abundance of thin-walled cells in stem cell wall,suggesting that OsCNGC10 was associated with the composition of stem cell wall and negatively regulated lodging resistance in rice.T2 generation field experiment indicated that compared with the wild type,oscngc10-2 significantly increased plant height,the effective panicle length,panicle number,seed setting rate,1000-grain weight,and yield per plant.The results of drought stress at seedling stage showed that malondialdehyde(MDA)content accumulated rap-idly in OsCNGC10 defective plants under drought stress and insufficient free proline(Pro)was formed,while the free Pro content in OsCNGC10 plants was significantly increased.Moreover,the MDA accumulation rate was relatively slow,which preliminarily indicated that OsCNGC10 positively regulated the drought resistance at seedling stage.The results of this study indicated that OsCNGC10 might have a potential function in lodging resistance and drought resistance in rice,which providing a theoretical basis and new germplasm resources for the breeding lodging resistance and high yield of new rice varieties.关键词
水稻/OsCNGC10/抗倒伏/抗旱/细胞壁Key words
rice/OsCNGC10/lodging resistance/drought tolerance/cell wall引用本文复制引用
朱忠林,文月,周棋,巫燕飞,杜雪竹,盛锋..水稻OsCNGC10基因抗倒伏性以及抗旱性功能研究[J].作物学报,2024,50(5):1351-1360,10.基金项目
本研究由武汉市生物技术关键技术科技重大专项(2022021302024851)和粮食作物种质创新与遗传改良湖北省重点实验室开放课题项目(2018lzjj01)资助.This study was supported by the Wuhan Municipal Key Technology Project of Biotechnology(2022021302024851)and the Hubei Provincial Key Laboratory of Grain Crop Germplasm Innovation and Genetic Improvement(2018LZJJ01). （2022021302024851）
