中国现代医生2024，Vol.62Issue(10)：39-42,4.DOI:10.3969/j.issn.1673-9701.2024.10.010
CAHB诱导成人急性淋巴细胞白血病Jurkat细胞凋亡机制的研究
Study on apoptosis mechanism of Jurkat cells induced by CAHB in adult acute lymphoblastic leukemia
张佳 1洪叶 1陈葆国 1罗文达1
作者信息
- 1. 浙江省台州医院血液内科,浙江临海 3170001
- 折叠
摘要
Abstract
Objective To explore the mechanism of apoptosis induced by diacetyl hexamethylene diamine(CAHB)in adult acute lymphoblastic leukemia Jurkat cells,and to provide theoretical basis for the clinical application of CAHB.Methods Annexin V+/PI-cell rate of Jurkat cells after CAHB induction was analyzed by flow cytometry.The Annexin V+/PI-cell rate was observed after treatment with caspase-9 inhibitor Z-LEHD-FMK.The expressions of apoptosis-related proteins caspase-8,caspase-9 and caspase-3 were observed by Western blotting.Results After CAHB induction,Jurkat cells were reduced in size,cell membrane crinkling,chromatin thickening,nuclear pyknosis or fragmentation,etc.Typical apoptotic bodies could be seen.CAHB induced Jurkat cell apoptosis by activating caspase-9 and caspase-3 in a dose-effect and time-dependent manner.Caspase-9 inhibitors could inhibit apoptosis induction of CAHB to a certain extent.Conclusion CAHB induced Jurkat cell apoptosis was related to caspase-9 and caspase-3 activation.关键词
二乙酰己二胺/急性淋巴细胞白血病/Jurkat细胞/凋亡Key words
Diacetyl hexamethylene diamine/Acute lymphoblastic leukemia/Jurkat cell/Apoptosis分类
医药卫生引用本文复制引用
张佳,洪叶,陈葆国,罗文达..CAHB诱导成人急性淋巴细胞白血病Jurkat细胞凋亡机制的研究[J].中国现代医生,2024,62(10):39-42,4.
