资源开发与市场2024，Vol.40Issue(4)：598-604,7.DOI:10.3969/j.issn.1005-8141.2024.04.012
麻疯树提取物活性物质及抗菌作用研究
Study on the active substances and antibacterial effects of extracts from Jatropha curcas L.
摘要
Abstract
In this study,ethanol-acetic acid(1:1)reflux technique was conducted to obtain J.curcas extract JE coumarins from leaves.Then,component quantification identification was measured via Liquid chromatography-mass spectrometry(LC-MS).The an-tibacterial function of coumarin compounds was verified by filter paper technique.Minimum Inhibitory Concentration(MIC)and Mini-mum Bactericidal Concentration(MBC)were measured via Broth microdilution and growth curve verification.As a result,the main component was Bergapten with 18.66％,which was firstly found in J.curcas extracts.Meanwhile,Baicalein(2.76％)and Rhoifolin(0.16％)were also discovered in the leaves extract.Antibacterial experiment resulted that coumarin could significantly suppress the growth of Staphylococcus aureus(S.anreus)and Escherichia coli(E.coli).The MIC of Jatropha curcas extracts to S.anreus and E.coli are 1.56 mg/mL and 3.125 mg/mL whereas the MBC are both 6.25mg/mL.关键词
麻疯树/香豆素类化合物/抑菌活性Key words
Jatropha curcas L./coumarins compounds/antibacterial activity分类
生物学引用本文复制引用
何静,李佛生,王晶金,高婷彦,颜钫,唐琳..麻疯树提取物活性物质及抗菌作用研究[J].资源开发与市场,2024,40(4):598-604,7.基金项目
凉山州科技转化项目(编号:21CGZH0004). （编号:21CGZH0004）
