中国现代中药2024，Vol.26Issue(3)：471-478,8.DOI:10.13313/j.issn.1673-4890.20230426001
基于UPLC指纹图谱与抗氧化谱效关系的枸杞子质量标志物研究
Quality Markers of Lycii Fructus Based on UPLC Fingerprint and Antioxidant Spectrum-effect Relationship
摘要
Abstract
Objective:To establish a method for the quality markers of Lycii Fructus based on ultra-high-performance liquid chromatography(UPLC)fingerprint and antioxidant spectrum-effect relationship,providing a basis for its quality evaluation.Methods:UPLC was used to establish fingerprint profiles for 31 batches of Lycii Fructus.Based on the common peak area results of the fingerprint profiles,cluster analysis,principal component analysis,and orthogonal partial least squares-discriminant analysis were used to evaluate the overall quality of Lycii Fructus and screen major quality markers.The antioxidant activity of Lycii Fructus was assessed by determining the values of multifunctional oxidases(MFO),1,1-diphenyl-2-picrylhydrazyl(DPPH),and total antioxidant capacity(T-AOC).The spectral-effect relationship between the common peaks in the fingerprint profiles and antioxidant activity indicators was studied using bivariate correlation analysis and orthogonal partial least squares-regression analysis to comprehensively select quality markers.Results:UPLC fingerprint profiles of Lycii Fructus identified 34 common peaks,and the 31 batches of Lycii Fructus were clustered into three categories,allowing for differentiation based on their origin.Principal component analysis revealed four primary factors influencing the classification of Lycii Fructus.Orthogonal partial least squares-discriminant analysis indicated that compounds in common peaks 2,7,17,19,and 33 could serve as primary quality markers for identifying and distinguishing Lycii Fructus.Bivariate correlation analysis showed that compounds in peaks 8,11,15,20,23,and 34 were highly correlated with the values of MFO,DPPH,and T-AOC.Orthogonal partial least squares-regression analysis indicated that compounds in peaks 11,15,23,25,31,33,and 34 constituted the major group of active components,which could serve as quality markers for the antioxidant activity of Lycii Fructus.Conclusion:The study elucidated the chemical basis of the antioxidant activity of Lycii Fructus and identified quality markers that can be used for quality control and assessment of Lycii Fructus.关键词
枸杞子/指纹图谱/质量标志物/超高效液相色谱法/化学模式识别/主成分分析Key words
Lycii Fructus/fingerprint/quality marker/ultra-high-performance liquid chromatography/chemical pattern recognition/principal component analysis分类
医药卫生引用本文复制引用
乔亚玲,宋霞,刘亚蓉,韩生兰,李永鹏,林鹏程..基于UPLC指纹图谱与抗氧化谱效关系的枸杞子质量标志物研究[J].中国现代中药,2024,26(3):471-478,8.基金项目
青海省科技计划项目(2020-SF-C32) （2020-SF-C32）
2021年度青海省"昆仑英才·高端创新创业人才"计划项目 （）
