中国现代中药2024，Vol.26Issue(3)：494-498,5.DOI:10.13313/j.issn.1673-4890.20230706004
基于薄层色谱与一测多评法的沙棘颗粒质量评价方法研究
Quality Evaluation of Shaji Granules Based on Thin Layer Chromatography and Quantitative Analysis of Multi-components by A Single Marker
摘要
Abstract
Objective:To optimize the thin layer chromatography(TLC)and establish a quantitative analysis of multi-components by a single marker(QAMS)method for Shaji Granules.Methods:Quercetin,isorhamnetin and kaempferol were qualitatively identified by TLC.A high-performance liquid chromatography(HPLC)was established using C18 column(250 mm×4.6 mm,5 µm)with acetonitrile-0.1%phosphoric acid solution as mobile phase in gradient elution at a flow rate of 1.0 mL·min-1.The column temperature was 35℃,and the detection wavelength was 365 nm.Isorhamnetin and kaempferol were determined with quercetin as internal reference.Results:TLC had good specificity and the spots were clear.Compared with the external standard method,the QAMS method was accurate and feasible.Conclusion:The optimized TLC and the established QAMS method were reliable,accurate and reproducible,which could provide a comprehensive reference for improving the quality control standards of Shaji Granules.关键词
沙棘颗粒/槲皮素/异鼠李素/山柰素/薄层色谱法/一测多评法Key words
Shaji Granules/quercetin/isorhamnetin/kaempferol/thin layer chromatography(TLC)/quantitative analysis of multi-components by a single marker(QAMS)分类
医药卫生引用本文复制引用
樊修和,唐志书,宋忠兴,王昌利,王珂..基于薄层色谱与一测多评法的沙棘颗粒质量评价方法研究[J].中国现代中药,2024,26(3):494-498,5.基金项目
陕西省重点研发计划项目(202190025) （202190025）
内蒙古自治区2019年度重大科技专项(2019ZD005) （2019ZD005）
