中国现代中药2024，Vol.26Issue(3)：528-534,7.DOI:10.13313/j.issn.1673-4890.20230802002
UPLC-MS/MS测定心悦胶囊中6个皂苷类成分的含量
Simultaneous Determination of Six Saponins in Xinyue Capsules by UPLC-MS/MS
摘要
Abstract
Objective:To establish the method for determination of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1,pseudo-ginsenoside F11,ginsenoside Rd and 20(S)-ginsenoside F1 in Xinyue Capsules by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods:The analysis was performed on Waters ACQUITY BEH C18 column(100 mm×2.1 mm,1.7 μm),with acetonitrile(A)-water(B)as the mobile phase,with gradient elution at a flow rate of 0.5 mL·min-1.The column temperature was set at 40℃.The analytes were detected using the electrospray ionization(ESI)source in multiple reaction monitoring(MRM)scan mode.Results:Six constituents showed good linear relationships within the concentration ranges of 9.638-963.800,9.839-983.900,9.951-995.100,5.270-1 054.000,9.608-960.800,4.905-981.000 ng·mL-1(r>0.998),whose average recoveries(RSD)were 96.9%(2.2%),100.1%(1.5%),97.2%(1.8%),98.5%(2.3%),96.6%(2.8%),100.2%(3.5%),respectively.Conclusion:The established method is rapid,accurate,and highly sensitive,making which can be used for quality control of total ginsenosides from Panax quinquefolium stems and leaves in Xinyue Capsules.关键词
超高效液相色谱-质谱法/心悦胶囊/西洋参茎叶总皂苷/人参皂苷Rg1/人参皂苷Re/人参皂苷Rb1/拟人参皂苷F11/人参皂苷Rd/20(S)-人参皂苷F1Key words
UPLC-MS/MS/Xinyue Capsules/total ginsenoside of Panax quinquefolium stems and leaves/ginsenoside Rg1/ginsenoside Re/ginsenoside Rb1/pseudo-ginsenoside F11/ginsenoside Rd/20(S)-ginsenoside F1分类
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赵振霞,王晓蕾,耿韫,朱靖,于新兰,刘永利..UPLC-MS/MS测定心悦胶囊中6个皂苷类成分的含量[J].中国现代中药,2024,26(3):528-534,7.基金项目
河北省重点研发计划项目(21372502D) （21372502D）
