遵义医科大学学报2024，Vol.47Issue(4)：324-331,8.
circGLS抑制非小细胞肺癌细胞恶性表型的作用研究
circGLS inhibits the malignant phenotype of non small cell lung cancer cells
摘要
Abstract
Objective To elucidate the circular RNA characteristics of circGLS,determine its circularization site,and investigate the impact of circGLS on the malignant phenotype of non-small cell lung cancer(NSCLC)cells.Methods Northern blot and RNase R resistance experiments were employed to assess the resistance of cir-cGLS to RNase R digestion,and Sanger sequencing determined its circularization site.H460 and H1299 cells were infected with Vector and OE-circGLS lentivirus,while A549 and H1975 cells were infected with sh-NC and sh-circGLC,resulting in Control(without infection of virus),Vector,OE-circGLS,sh-NC,and sh-circGLS groups.CCK-8,flow cytometry,scratch,and Transwell assays were conducted to investigate cell proliferation,apoptosis,migration,and invasion in the aforementioned groups.Results Northern blot,RNase R digestion ex-periments and Sanger sequencing confirmed the resistance of circGLS to RNase R digestion,with the circulariza-tion site identified as"GA".RT-qPCR results indicated successful construction of the cell models.CCK-8 ex-periments showed that the cell viability of H1299 and H460 cells in OE-circGLS group at 72 h was 0.88 and 0.9,respectively,while the viability of A549 and H1975 cells in sh-circGLS at 72 h was 1.13(normalized to 1 for Vector and sh-NC groups).Scratch experiments revealed healing rates of H460 and H1299 cells in the OE-circGLS group vs Vector group=2.42％vs 5.57％and 12.09％vs 25.07％,respectively.Healing rates for A549 and H1975 cells in the sh-circGLS group vs sh-NC group=19.00％vs 13.81％and 30.69％vs 21.53％,respectively.Transwell experiments showed migration rates(OD)of H460 and H1 299 cells in the OE-circGLS group vs Vector group=0.94 vs 1.01 and 0.57 vs 0.92,respectively.Migration rates for H1975 and A549 cells in the sh-circGLS group vs sh-NC group=1.044 vs 0.95 and 1.29 vs 1.09,respectively.Flow cytometry results indicated apoptosis rates of H1299 and H460 cells in the OE-circGLS group vs Vector group=14.45％vs 7.54％and 10.74％vs 1.79％,respectively.Apoptosis rates of A549 and H1975 cells in the sh-circGLS group vs sh-NC group=8.57％vs 9.52％and 2.47％vs 4.95％,respectively.These results suggest that overexpression of circGLS significantly inhibits cell proliferation,migration,and invasion in NSCLC,while its overexpression promotes apoptosis.Knockdown of circGLS presented the opposite results.Conclusion It was determined that circGLS is a circular RNA which could suppress the malignant phenotype of NSCLC cells.Con-sequently,circGLS emerges as a promising candidate for therapy of NSCLC.关键词
环状RNA/circGLS/非小细胞肺癌/恶性表型Key words
circular RNA/circGLS/non small cell lung cancer/malignant phenotype分类
医药卫生引用本文复制引用
龙开君,赵鹏飞,王凯,宋永祥,陈成,柯希贤..circGLS抑制非小细胞肺癌细胞恶性表型的作用研究[J].遵义医科大学学报,2024,47(4):324-331,8.基金项目
国家自然科学基金资助项目(NO:81960532) （NO:81960532）
贵州省科技计划资助项目[NO:黔科合基础-ZK(2022)一般644] （2022）
遵义医科大学附属医院博士科研启动基金[NO:院字(2019)05号]. （2019）
