中药联合针刺对脾虚型胃癌前期病变模型大鼠的实验研究OA
Experimental Study on the Effect of Traditional Chinese Medicine Combined with Acupuncture on Pre-Gastric Cancer Model Rats
目的:探究中药联合针刺对脾虚型胃癌前期病变模型大鼠的影响.方法:选取120只成年SD大鼠,随机分为对照组、模型组、中药组和联合组,每组30只.除对照组外,其余各组大鼠采用N-甲基-N′-硝基-亚硝基胍综合造模法复制脾虚型胃癌前期病变模型.中药组灌胃5.4 g/kg中药混悬液2 mL,联合组采用中药灌胃联合针刺治疗,对照组和模型组给予等量生理盐水灌胃,均治疗3周.观察大鼠体质量、24 h进食量和脏器系数的变化情况;检测大鼠外周血中TCRαβ+CD161a+、CD4+及CD4+/CD8+含量;采用电化学发光法检测大鼠血浆中CEA、CA199、CA724的水平;检测大鼠胃组织中TLR-NF/κB信号通路蛋白表达.结果:与对照组相比,模型组大鼠体质量、24 h进食量、脾脏指数、胸腺指数、TCRαβ+CD161a+、CD4+比例、CD4+/CD8+比值均显著降低(P<0.05),血清中CD8+比例、CEA水平、CA199水平、CA724水平、胃组织中TLR-4和NF-κB p65蛋白相对表达水平均显著升高(P<0.05).与模型组相比,中药组和联合组大鼠体质量、24 h进食量、脾脏指数、胸腺指数、TCRαβ+CD161a+、CD4+比例、CD4+/CD8+比值均显著升高(P<0.05),血清中CD8+比例、CEA水平、CA199水平、CA724水平、胃组织中TLR-4和NF-κB p65蛋白相对表达水平均显著降低(P<0.05).与中药组比较,联合组24 h进食量、脾脏指数、胸腺指数、CD4+水平均显著升高(P<0.05),血清中CEA水平、CA199水平、CA724水平、胃组织中TLR-4和NF-κB p65蛋白相对表达水平显著降低(P<0.05).结论:中药联合针刺可以降低脾虚型胃癌前期病变模型大鼠血清肿瘤标志物水平,并调节T淋巴细胞和NK细胞活性,增强机体免疫调节,其作用机制可能与调控TLR-NF/κB信号通路相关.
Objective:To explore the effect of traditional Chinese medicine combined with acupuncture on pre-gastric cancer model rats.Methods:A hundred and twenty adult SD rats were randomly divided into control group,model group,traditional Chinese medicine group,and combination group,with 30 rats in each group.Except for the control group,the other groups were treated with N-methyl-N′-nitroso-guanidine comprehensive modeling method to create the spleen deficiency type pre-gastric cancer model.The traditional Chinese medicine group was gavaged with 5.4 g/kg traditional Chinese medicine suspension 2 ml;the combination group was gabaged with traditional Chinese medicine combined with acupuncture;and the control group and model group were given the same amount of normal saline in the same way,for 3 weeks.The changes of body mass,24 h food intake and organ coefficient were observed.The contents of TCRαβ+CD161a+,CD4+and CD4+/CD8+in peripheral blood of rats were detected.The levels of CEA,CA199 and CA724 in rat plasma were detected by electrochemiluminescence method.The expression of TLR-NF/κB signaling pathway protein in rat stomach was detected.Results:Compared with the control group,body mass,24 h food intake,spleen index,thymus index,TCRαβ+CD161a+,CD4+ratio and CD4+/CD8+ratio of rats in model group were significantly decreased(P<0.05);and the proportion of serum CD8+,CEA level,CA199 level,CA724 level,and the relative expression levels of TLR-4 and NF-κB p65 protein in gastric tissue were significantly increased(P<0.05).Compared with model group,body mass,24 h food intake,spleen index,thymus index,TCRαβ+CD161a+,CD4+ratio and CD4+/CD8+ratio of rats in traditional Chinese medicine group and combination group were significantly increased(P<0.05);while the proportion of serum CD8+,CEA level,CA199 level,CA724 level,and the relative expression levels of TLR-4 and NF-κB p65 protein in gastric tissue were significantly decreased(P<0.05).Compared with traditional Chinese medicine group,24 h food intake,spleen index,thymus index and CD4+level in combination group were significantly increased(P<0.05),while the levels of CEA,CA199 and CA724 in serum and the relative expression levels of TLR-4 and NF-κB p65 in gastric tissue were significantly decreased(P<0.05).Conclusion:Traditional Chinese medicine combined with acupuncture can reduce the level of serum tumor markers,regulate the activity of T lymphocytes and NK cells,and enhance immune regulation in rats with splen deficiency type precancerous gastric cancer,and its mechanism may be related to the regulation of TLR-NF/κB signaling pathway.
阳国彬;李亚晨;刘玉芳;李佳;邹莹;李宝华;陈艳;张荔;宇金美;龚晨
襄阳市中医医院,湖北 襄阳 441000湖北中医药大学,湖北 武汉 430065
针刺胃癌肿瘤标志物TLR-NF/κB信号通路
AcupunctureGastric cancerTumor markersTLR-NF/κB signaling pathway
《中医药信息》 2024 (004)
40-44,54 / 6
湖北省自然科学基金面上项目(2022CFB367)
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