广西医科大学学报2024，Vol.41Issue(4)：540-547,8.DOI:10.16190/j.cnki.45-1211/r.2024.04.009
RASA4表达失活促进鼻咽癌增殖的机制研究
Mechanism of RASA4 expression inactivation promoting proliferation of nasopharyngeal car-cinoma
摘要
Abstract
Objective:To explore the expression of RASA4gene in nasopharyngeal carcinoma(NPC),and to in-vestigate the influence and molecular mechanism of RASA4gene affecting the proliferation of NPC.Methods:Meta-analysis of RASA4transcription levels in NPC was performed using GEO database.RASA4mRNA and protein expression levels in NPC cell lines were detected by reverse transcription-quantitative PCR(RT-qPCR)and western blotting.RASA4protein expression and localization in NPC tissues were detected using immunohis-tochemistry(IHC).The impact of RASA4on cell proliferation was evaluated through cell counting kit-8(CCK-8)assay and colony formation assay.Results:Bioinformatics analysis based on GEO data showed that RASA4 mRNA expression was significantly down-regulated in NPC.RT-qPCR and western blotting results indicated that RASA4 was down-regulated at both mRNA and protein expression in NPC cells.Exogenous overexpression of the RASA4gene could inhibit the cell proliferation of NPC.Conclusion:RASA4may serve as a potential sup-pressor gene in NPC,and investigating its inactivation mechanism and function is of significant importance for the pathogenesis and treatment of NPC.关键词
鼻咽癌/RASA4/细胞增殖Key words
nasopharyngeal carcinoma/RASA4/cell proliferation分类
医药卫生引用本文复制引用
汤诗玥,赵军,张海山,李丽媚,周晓莹,黄议莹,温文胜..RASA4表达失活促进鼻咽癌增殖的机制研究[J].广西医科大学学报,2024,41(4):540-547,8.基金项目
国家自然科学基金资助项目(No.81860479 （）
No.81960490) （）
区域性高发肿瘤早期防治研究教育部重点实验室/广西重点实验室自主课题(No.GKE-ZZ 202145) （No.GKE-ZZ 202145）
