广西医科大学学报2024，Vol.41Issue(4)：558-563,6.DOI:10.16190/j.cnki.45-1211/r.2024.04.011
钯锌合金纳米酶对炎症软骨细胞模型的影响
Effect of palladium-zinc alloy nanozymes on an inflammatory chondrocyte model
摘要
Abstract
Objective:To discuss the anti-inflammatory effect of palladium-zinc alloy(PdZn/C)nanozymes on an inflammatory chondrocyte model induced by hydrogen peroxide(H2O2).Methods:The PdZn/C nanozymes were synthesized and characterized.The PdZn/C nanozymes were assayed for simulated superoxide dismutase ac-tivity(SOD)and simulated catalase activity(CAT).The biocompatibility of PdZn/C nanozymes with chondro-cytes was examined in vitro by cell counting kit-8(CCK-8)assay.The H2O2-induced inflammatory chondrocytes were co-cultured with PdZn/C nanozymes,the reactive oxygen species(ROS)levels of inflammatory chondro-cytes were assessed by ROS fluorescent probe,and immunofluorescence assay was performed to detect the pro-tein expression levels of interleukin(IL)-1β and tumor necrosis factor(TNF)-α in inflammatory chondrocytes.Re-sults:The successful synthesis of PdZn/C nanozymes was confirmed by transmission electron microscopy(TEM),X-ray diffraction(XRD)and zeta potential.The results of CCK-8 assay confirmed that PdZn/C nano-zymes had good biocompatibility.The PdZn/C nanozymes had excellent SOD and CAT mimicking activities.The PdZn/C nanozymes were able to reduce the ROS levels as well as the expression of IL-1β and TNF-α proteins in the inflammation model.Conclusion:The PdZn/C nanozymes have good biocompatibility and can reduce the in-flammatory response of chondrocytes,making them a promising anti-inflammatory nanomaterial.关键词
钯锌合金/纳米酶/活性氧/骨关节炎Key words
palladium-zinc alloy/nanozymes/reactive oxygen species/osteoarthritis分类
医药卫生引用本文复制引用
向剑辉,苏永峰,唐斌,蒙国色,程建文..钯锌合金纳米酶对炎症软骨细胞模型的影响[J].广西医科大学学报,2024,41(4):558-563,6.基金项目
国家自然科学基金资助项目(No.81960400) （No.81960400）
