口腔疾病防治2024，Vol.32Issue(6)：411-419,9.DOI:10.12016/j.issn.2096-1456.2024.06.002
滑膜细胞及软骨细胞释放HMGB1流体力学阈值的研究
Investigation of the hydromechanical threshold of high-mobility group box 1 release from synovial cells and chondrocytes
摘要
Abstract
Objective To investigate the effect of fluid flow shear stress(FFSS)on the fluid mechanic threshold of high-mobility group box 1(HMGB1)release by synovial cells and chondrocytes.Moreover,the mechanism of chondro-cyte and synovial cell damage induced by abnormal mechanical force was investigated to provide an experimental basis for exploring the pathogenesis and pathology of temporomandibular joint osteoarthritis.Methods With the approval of the Ethics Committee for Animal Experiments of the hospital,synovial tissue and cartilage tissue blocks were obtained from the knee joints of Sprague-Dawley(SD)rats,and synovial cells and chondrocytes were cultured and digested for subsequent experiments.Synovial cells and chondrocytes of 3-4 generations were acquired,and FFSS was applied to sy-novial and cartilage cells using a fluid shear mechanical device.The cells were divided according to the FFSS values of different sizes.Synovial cells were stimulated for 1 h with 1,3,5,or 10 dyn/cm2 of FFSS,and chondrocytes were stimu-lated for 1 h with 4,8,12,or 16 dyn/cm2 of FFSS.Resting cultures(0 dyn/cm2)were used as the control group.Changes in the morphology of the cells were observed.The expression and distribution of HMGB1 and interleukin-1β(IL-1β)were observed by immunohistochemistry.The expression of HMGB1 and IL-1β in the supernatant was analyzed by ELI-SA.The protein expression levels of intracellular HMGB1 and IL-1β were detected by Western blot.Results With in-creasing FFSS,the synovial cells and chondrocytes gradually swelled and ruptured,and the number of cells decreased.With increasing FFSS,the localizationof HMGB1 and IL-1β gradually shifted from the nucleus to the cytoplasm.In synovial cells,compared with those in the control group,the expression levels of HMGB1 and IL-1β were increased both in the supernatant and cells in the 1,3,5 and 10 dyn/cm2 intervention groups(P＜0.01).In chondrocytes,com-pared with those in the control group,the expression levels of HMGB1 in the supernatant were increased in the 4,12 and 16 dyn/cm2 intervention groups(P＜0.05),and the protein expression levels of HMGB1 were significantly increased(P＜0.01).The expression levels of HMGB1 in the supernatant were significantly increased in the 8 dyn/cm2 intervention groups(P＜0.01);however,the protein expression levels of HMGB1 were significantly decreased.Compared with those in the control group,the expression levels of IL-1β in the supernatant gradually increased in the 4,8,12 and 16 dyn/cm2 intervention groups(P＜0.01).With the exception of those in the 4 dyn/cm2 group,the protein expression levels of IL-1β gradually increased with increasing FFSS(P＜0.05).Conclusion With increasing FFSS,synovial cells and chondro-cytes gradually swelled and burst,and the hydromechanical thresholds of HMGB1 release were 1 dyn/cm2 and 8 dyn/cm2,respectively.Therefore,upon stimulation with a mechanical force,synovial damage was damaged before chondrocytes.关键词
颞下颌关节骨关节炎/滑膜细胞/软骨细胞/流体流动剪切力/阈值/高迁移率族蛋白1/白细胞介素-1βKey words
temporomandibular joint osteoarthritis/synovial cells/chondrocytes/fluid flow shear stress/threshold/high-mobility group box 1/interleukin-1β分类
医药卫生引用本文复制引用
童乔莹,邵博,许颖捷,贾梦莹,马优祎,龚忠诚..滑膜细胞及软骨细胞释放HMGB1流体力学阈值的研究[J].口腔疾病防治,2024,32(6):411-419,9.基金项目
国家自然科学基金(82160189,82260196) （82160189,82260196）
新疆维吾尔自治区自然科学基金(2022D01C480) （2022D01C480）
新疆维吾尔自治区自治区自然科学基金重点项目(2023D01D14) This study was supported by the grants from National Natural Science Foundation of China(No.82160189,No.82260196) （2023D01D14）
Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2022D01C480) （No.2022D01C480）
Major Project of Xinjiang Uygur Autonomous Region Natural science Foundation(No.2023D01D14). （No.2023D01D14）
