华北农学报2024,Vol.39Issue(2):19-26,8.DOI:10.7668/hbnxb.20194516
谷子基因组中SSR的偏态分布
Preference Distribution of Simple Sequence Repeats in Foxtail Millet Genome
摘要
Abstract
To reveal the distribution preferences of SSR in foxtail millet genome,the study analyzed distribu-tion regularities of SSRs among the 5'-flanking regions,the 3'-flanking regions,the extron regions and the intron re-gions in gene locus from foxtail millet genome using bioinformatics methods.The results showed that the relative fre-quency,a ratio of the frequency of a type of SSR in a certain genomic region by the frequency of this SSR in whole genome,of SSRs decreased with increasing of the distance of 5'-flanking regions from start codon,and was more than 1.50 obviously in a range from 1 bp to 700 bp.However,the relative frequency of SSRs was close to 1.00 in each of other three regions.Furthermore,with increasing of the distance of 5'-flanking regions from start codon,the relative frequency of various SSR types revealed the various changing trends,and as a whole,6 SSR types,including CCG,AG,AGG,AGGG,ACC and AGC,showed both the high frequency and the high relative frequency,and of these 6 types,the distance range(1-1800 bp)of AG,in which its relative frequency was more than 1.50,is the maximum while that of CCG is minimal(1-200 bp).Meanwhile,in the extron regions,5 SSR types with high C/G contents,in-cluding CCG,AGG,AGC,ACG and ACC,showed both a high frequency and a high relative frequency.It was conclu-ded that SSRs distributed preferentially in a length range from about 1-700 bp of 5'-flanking regions with 6 main spe-cific SSR types,including CCG,AG,AGG,AGGG,ACC and AGC;on the other hand,in 3'-flanking regions,the intron regions and the extron region,distribution of SSR is close to that in genome,and however,in the extron region,a total of 5 SSR types,including CCG,AGG,AGC,ACG and ACC,are obviously specifically distributed.关键词
谷子/SSR/分布偏好/5'侧翼区/外显子区Key words
Foxtail millet/SSR/Distribution preference/5'-flanking regions/Extron regions分类
农业科技引用本文复制引用
高建明,邱丽娜,桂枝..谷子基因组中SSR的偏态分布[J].华北农学报,2024,39(2):19-26,8.基金项目
国家自然科学基金(32301817) (32301817)
天津市自然科学基金(22JCQNJC01470) (22JCQNJC01470)