华北农学报2024，Vol.39Issue(2)：62-70,9.DOI:10.7668/hbnxb.20194583
球茎甘蓝MYB62转录因子的克隆与表达特征分析
Cloning and Expression Analysis of MYB62 Transcription Factors from Kohlrabi
摘要
Abstract
In order to clarify the sequence characteristics of the MYB62 transcription factor,expression changes after stress,and further explore the biological function of the MYB62 transcription factor,kohlrabis were used as plant materials.MYB62 transcription factors were obtained by homologous cloning method and bioinformatics was analyzed.Spatiotemporal expression analysis of MYB62 and its expression after stress were performed by Real-time PCR.Results of gene cloning showed that gDNA length of BocMYB62 gene was 1353 bp,the length of CDS was 837 bp,which contained four exons and three introns and encoded 278 amino acids.The sequence structure analysis showed that BocMYB62 was a hydrophilic protein with two SANT-MYB domains,belonging to the R2R3-MYB type in the MYB transcription factor family.The spatial structure prediction revealed a typical α-helix structure.Phyloge-netic analysis indicated that BocMYB62 was closely related to MYB62 in Brassica napus.The spatiotemporal expres-sion results showed that BocMYB62 was consistently higher in green kohlrabi than that in purple with clear tissue spe-cificity.The expression of BocMYB62 was significantly increased during drought stress with the highest expression un-der 12 h.The BocMYB62 expression was significantly lower than control and the lowest at 4℃ cold stress.These re-sults inferred that BocMYB62 may be involved in the regulation of anthocyanin biosynthesis and the regulation of stress.It provides a theoretical basis for the subsequent functional identification of MYB62 transcription factors.关键词
球茎甘蓝/MYB62/序列分析/保守基序/亲缘关系/荧光定量PCRKey words
Kohlrabi/MYB62/Sequence analysis/Conversed motifs/Relationship/Real-time PCR分类
农业科技引用本文复制引用
王鑫淼,赵孟良,邵登魁,尕桑,任延靖..球茎甘蓝MYB62转录因子的克隆与表达特征分析[J].华北农学报,2024,39(2):62-70,9.基金项目
国家大宗蔬菜产业技术体系项目(CARS-23-G26) （CARS-23-G26）
科技成果转化专项(2023-NK-144) （2023-NK-144）
青海省"高端创新人才千人计划"项目(K9923197) （K9923197）
