华北农学报2024，Vol.39Issue(2)：71-78,8.DOI:10.7668/hbnxb.20194438
防风SAUR32/23基因生物信息学分析及编码蛋白的亚细胞定位
Bioinformatics Analysis and Subcellular Localization of SAUR32/23 Genes in Saposhnikovia divaricata
摘要
Abstract
The bioinformatic analysis of SAUR genes aims to unveil their structure,function,and evolutionary relationships.Simultaneously,through subcellular localization,the goal was to understand their position within the cell,thereby inferring their biological functions.Such analyses contribute to a deeper understanding of the gene's mechanistic role and potential applications within an organism.This held particular theoretical and practical signifi-cance for regulating the germination of windproof seeds,reducing seed dormancy duration,and facilitating green cul-tivation and breeding of wind-resistant plants.In order to explore the biological characteristics and functions of SAUR genes,SdSAUR32 and SdSAUR23 were cloned from Saposhnikovia divaricata,and bioinformatics analysis was conducted to analyze the expression and subcellular localization of these two genes in different dormancy stages of Saposhnikovia divaricata seeds.The findings indicated that the SdSAUR32 protein had a molecular weight of ap-proximately 14.48 ku(pI 6.45),while the SdSAUR23 protein had a molecular weight of around 10.29 ku(pI 8.00),confirming that both proteins were hydrophilic.The conserved domain analysis of SdSAURs gene en-coded protein showed that SdSAUR32 and SdSAUR23 had the same conserved domain,which belonged to auxin induced superfamily members.The phylogenetic analysis showed that both SdSAUR32 and SdSAUR23 encoded protein was the closest relative to Daucus carota.The subcellular localization results indicated that both Sd-SAUR32 and SdSAUR23 were found within the nucleus.The SdSAUR32 and SdSAUR23 genes were found to be expressed during different stages of Saposhnikovia divaricata seed development.It was observed that the expression levels of these two genes were significantly higher during the germinal stage compared to the release dormancy stage and dormancy stage.This suggested that SdSAUR32 and SdSAUR23 may have a crucial role in the germination,growth,and development of Saposhnikovia divaricata seeds.This study offers not only insights for investigating the role of the SAUR gene family but also establishes atheoretical framework for examining the function of other genes in the parsnip family at the molecular level.关键词
防风/SAUR/生物信息学/亚细胞定位Key words
Saposhnikovia divaricata/SAUR/Bioinformatics/Subcellular localization分类
生物科学引用本文复制引用
曹正林,陈福源,黄玉兰,李有真,丁洪霞,夏凯旋,魏文琦..防风SAUR32/23基因生物信息学分析及编码蛋白的亚细胞定位[J].华北农学报,2024,39(2):71-78,8.基金项目
国家级大学生创新创业训练项目(202210223049X) （202210223049X）
黑龙江省自然科学基金联合引导项目(LH2023C077) （LH2023C077）
