Collaborative exercise:analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platformOA
Collaborative exercise:analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform
Human age estimation from trace samples may give important leads early in a police investigation by contributing to the description of the perpetrator.Several molecular biomarkers are available for the estimation of chronological age,and currently,DNA methylation patterns are the most promising.In this study,a QIAGEN age protocol for age estimation was tested by five forensic genetic laboratories.The assay comprised bisulfite treatment of the extracted DNA,amplification of five CpG loci(in the genes of ELOVL2,C1orf132,TRIM59,KLF14,and FHL2),and sequencing of the amplicons using the PyroMark Q48 platform.Blood samples from 49 individuals with ages ranging from 18 to 64 years as well as negative and methylation controls were analyzed.An existing age estimation model was applied to display a mean absolute deviation of 3.62 years within the reference data set.
Marie-Louise Kampmann;Bruce McCord;Peter M.Schneider;Jiri Drabek;Niels Morling;Jan Fleckhaus;Claus Børsting;Helena Jurtikova;Alice Piters;Julien Papin;Quentin Gauthier;Mirna Ghemrawi;Christian Doutremepuich
Section of Forensic Genetics,Department of Forensic Medicine,Faculty of Health and Medical Sciences,University of Copenhagen Copenhagen,DenmarkDepartment of Chemistry and Biochemistry,Florida International University,Miami,FL,USAInstitute of Legal Medicine,Faculty of Medicine and University Clinic,University of Cologne,Cologne,GermanyInstitute of Molecular and Translational Medicine,Faculty of Medicine and Dentistry,Palacky University Olomouc and the University Hospital Olomouc,Olomouc,the Czech RepublicLaboratoire d'Hématologie Médico-Légale,Bordeaux Cedex,France
forensic geneticsage estimationDNA methylationpyrosequencingtrace sample
《法庭科学研究（英文）》 2024 (001)
84-89 / 6
This work was supported by LM2018125 for the work per-formed in Palacky University,Czech Republic;by the National Institute of Justice,Department of Justice,USA under grant 2017-NE-BX-0001 for work performed at the Department of Chemistry and Biochemistry,Florida International University,Miami,FL,USA.Points of view in the document are those of the authors and do not necessarily represent the official view of the US Department of Justice.
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