海南医学院学报2024，Vol.30Issue(8)：561-567,7.DOI:10.13210/j.cnki.jhmu.20240017.001
M2型巨噬细胞通过分泌白细胞介素-10调控胆囊癌细胞增殖、迁移和侵袭的研究
M2 macrophages regulate the proliferation,migration and invasion of gallbladder cancer cells by secreting interleukin-10
摘要
Abstract
Objective:To investigate the effect of M2 macrophages on the proliferation,migration and invasion of gallbladder carcinoma cells by regulating the expression of interleukin-10(IL-10).Methods:Human monocyte line THP-1 was induced into M2 macrophages in vitro,and the expression of corresponding markers and IL-10 was detected by RT-qPCR.The expression of IL-10 in cell supernatant was detected by ELISA.GBC cell lines NOZ and GBC-SD were divided into the Control group(serum-free medium)、the M2-CM group(M2 macrophage conditioned medium)、the M2-CM+anti-IL-10 group(M2-CM containing anti-IL-10)and the M2-CM+IgG group(M2-CM containing IL-10 isotype control antibody).The cell proliferation rate of each group was measured by CCK-8 test,the cell migration rate of each group was detected by cell scratch test and the change in cell in-vasive ability of each group was detected by Transwell test;and Western blot assay was used to detect the expression of epithelial-mesenchymal transformation markers(E-cadherin and Vimentin)in each group.Results:M2 macrophages were successfully in-duced in vitro.Compared with uninduced THP-1,IL-10 was highly expressed in M2 macrophages and M2 macrophages superna-tant(P<0.05).M2 macrophages could promote the proliferation,migration and invasion of GBC cells,as well as inhibit the ex-pression of E-cadherin and promote the expression of Vimentin.Blocking IL-10 could inhibit the effect of M2 macrophages(P<0.05).Conclusion:M2 macrophages can promote the proliferation,migration,invasion and epithelial-mesenchymal transforma-tion of GBC cells by secreting IL-10.关键词
胆囊癌/M2型巨噬细胞/白细胞介素-10/增殖/迁移/侵袭Key words
Gallbladder carcinoma/M2 macrophage/Interleukin-10/Proliferation/Migration and invasion分类
医药卫生引用本文复制引用
赵向阳,江博文,陶滔,谈燚..M2型巨噬细胞通过分泌白细胞介素-10调控胆囊癌细胞增殖、迁移和侵袭的研究[J].海南医学院学报,2024,30(8):561-567,7.基金项目
This study was supported by Major Natural Science Research Projects in Colleges and Universities in Anhui Province(KJ2018ZD022) （KJ2018ZD022）
Key Projects of Education Department of Anhui Province(2022AH051416) （2022AH051416）
Graduate Scientific Research and Innovation Program of Bengbu Medical College(Byycx22089) 安徽省高校自然科学研究重大项目(KJ2018ZD022) （Byycx22089）
安徽省教育厅重点项目(2022AH051416) （2022AH051416）
蚌埠医学院研究生院科研创新项目(Byycx22089) （Byycx22089）
