高校化学工程学报2024，Vol.38Issue(2)：253-264,12.DOI:10.3969/j.issn.1003-9015.2024.02.010
半疏水晶胶微球的悬浮特性及固载乳杆菌合成有机酸
Suspension characteristics of semi-hydrophobic cryogel beads and their application in Lactobacillus paracasei cell immobilization for organic acid production
摘要
Abstract
In order to solve the problems of substrate and product inhibition,low cell density and organic acid tolerance,poly(hydroxyethyl methacrylate-butyl methacrylate)semi-hydrophobic cryogel beads were prepared by"crystallization pore-forming and low temperature polymerization",and their suspension behaviors in a 10 L stirred bioreactor were investigated by high-speed imaging and numerical simulation.Lactic acid and phenyllactic acid biosynthesis processes were studied using these cryogel bead immobilized Lactobacillus.The results show that the suspension characteristics of the cryogel beads obtained by numerical simulation are consistent with those of high-speed imaging,and the minimal stirring rate required for uniform suspension of cryogel beads(volume fraction is 3%)is 55 r·min-1.The Lactobacillus biomass reached 10.5 g·L-1 in the co-production process of lactic acid and phenyllactic acid,which was increased by 84.2%compared with Lactobacillus fermentation without immobilization.During the first feeding period,the fermentation period of lactic acid was greatly shortened to 21 h,which was 50%shorter than that without immobilization.关键词
半疏水晶胶微球/搅拌式生物反应器/悬浮行为/乳杆菌/发酵/数值模拟Key words
semi-hydrophobic cryogel bead/stirred tank bioreactor/suspension behavior/Lactobacillus/fermentation/numerical simulation分类
化学化工引用本文复制引用
侯东杰,楼小玲,蔡礼年,陈治宏,张玮,陈冰冰,贠军贤..半疏水晶胶微球的悬浮特性及固载乳杆菌合成有机酸[J].高校化学工程学报,2024,38(2):253-264,12.基金项目
浙江省自然科学基金重大项目(LD21B060001) （LD21B060001）
国家自然科学基金(22078296,21576240). （22078296,21576240）
