森林与环境学报2024，Vol.44Issue(3)：289-297,9.DOI:10.13324/j.cnki.jfcf.2024.03.009
闽楠挥发性组分提取工艺优化及组学分析
Optimized extraction and metabolomics analysis of volatile components in Phoebe bournei
摘要
Abstract
In order to optimize the steam distillation process of volatile components in Phoebe bournei and explore the differences,four parts(leaves,branches,trunks and roots)from the P.bournei test forest in Lechang City,Guangdong Province were used as materials to conduct single-factor tests and orthogonal tests by steam distillation,aims to investigate the influence of three factors(NaCl concentration,material-liquid ratio to solution and extraction time)on the yield of volatile components,determine the optimum process parameters.The extracts from four parts of P.bournei were determined by GC-MS to explore the content and differences of volatile components.The results showed that:(1)the optimal process combinations for leaves was NaCl concentration 45 g·L-1,material-liquid ratio 1∶6,and extraction time 5 h.The extraction rate was 0.056%,110 volatile components detected.The top five fractions were beta-selinene,beta-eudesmol,bicyclogermacrene,aromandendrene and alpha-muurolene.(2)the optimal process combinations for branches was NaCl concentration 45 g·L-1,material-liquid ratio 1∶8,and extraction time 5 h.The extraction rate was 0.083%,80 volatile components detected.The top five fractions were guaiol,guaiol acetate,agarospirol,gamma-eudesmol and delta-cadinene.(3)the optimal process combinations for trunks was NaCl concentration 30 g·L-1,material-liquid ratio 1∶8,and extraction time 5 h.The extraction rate was 0.323%,64 volatile components detected.The top five fractions were agarospirol,gamma-cadinene,guaiol,gamma-eudesmol and verbenone.(4)the optimal process combinations for roots was NaCl concentration30 g·L-1,material-liquid ratio 1∶9,and extraction time 5 h.The extraction rate was 1.025%,69 volatile components detected.The top five fractions were agarospirol,gamma-cadinene,guaiol,gamma-eudesmol and verbenone.(5)these volatile components were major consisted of terpenes and alcohols,accounting for more than 80%of the total content.Only 15 volatile components were common among leaves,branches,trunks and roots.The composition of volatile components separated from leaves was significantly different compared with these from other materials.关键词
闽楠/挥发性组分/提取工艺/水蒸气蒸馏法/正交试验/气相色谱-质谱联用(GC-MS)/代谢组学Key words
Phoebe bournei/volatile components/extraction process/steam distillation/orthogonal test/GC-MS/metabolomics分类
农业科技引用本文复制引用
刘桐语,李荣生,杨锦昌,袁在翔,余纽,吴伟煌..闽楠挥发性组分提取工艺优化及组学分析[J].森林与环境学报,2024,44(3):289-297,9.基金项目
中国林业科学研究院中央级公益性科研院所基本科研业务费专项"沉香、猴耳环等树种功能成分分析与质量评价"(CAFYBB2020SY019). （CAFYBB2020SY019）
