动物医学进展2024，Vol.45Issue(5)：18-24,7.
体外抗猪轮状病毒8种藏药的初步筛选
Preliminary Screening of 8 Tibetan Medicines against Porcine Rotavirus in vitro
摘要
Abstract
In order to screen Tibetan medicines with antiviral effects,in vitro antiviral studies are carried out on extracts of eight Tibetan medicines,including Terminalia chebula,Aconitum tanguticum,Corydalis edulis,Dracocephalum tanguticum,Swertia mussotii,Lagotis brachystachya,Aconitum pendulum,Stellera chamaejasme,which are found to have potential antiviral effects.Eight Tibetan medicines were extracted with water.Water extracts of different concentrations were prepared by concentrating and vacu-um drying.Porcine rotavirus(PoRV)was used as the test object.The safe concentration of the medicines was determined by CCK-8 and the anti-porcine rotavirus effect of eight medicines in vitro was determined by the combination of cytopathic effect(CPE)observation,real-time quantitative PCR and indirect immunofluorescence assay.At the same time,ribavirin was used as an antiviral control.The result showed that the maximum safe concentrations of eight Tibetan medicinal extracts of Terminalia chebula,Aconitum tanguticum,Dracocephalum tanguticum,Lagotis brachystachya,Swertia mussotii,Aconitum pendulum,Stellera chamaejasme,Corydalis edulis and ribavirin were 0.015 mg/mL,0.49 mg/mL,0.98 mg/mL,2.60 mg/mL,7.81 mg/mL,15.63 mg/mL,1.30 mg/mL,31.25 mg/mL and 0.20 mg/mL,respectively.Antiviral tests showed that after the medicinal interactions with the viruses,the maximum inhibitory rates of Swertia mussotii,Stellera chamaejasme,Lagotis brachystachya,Aconitum pendulum,Terminalia chebula and ribavirin against porcine rotavirus were all over 50％,which were 87.37％,91.55％,86.19％,91.88％,63.74％and 96.33％,respectively.It could effectively reduce the cytopathic effect caused by viral infection.The maximum inhibitory rates of the other three extracts on PoRV cyto-pathic effect was lower than 20％.Further tests on the five Tibetan medicines with the maximum inhibition rate of more than 50％of PoRV showed that the five Tibetan medicine extracts significantly reduced the a-bility of virus replication(P＜0.05)and effectively reduced the infections of virus in MA-104 cells.This study revealed that five extracts of Tibetan medicines have certain antiviral effects on PoRV,which pro-vides basic data for screening and mechanism research of antiviral Tibetan medicines.关键词
藏药/猪轮状病毒/抗病毒/体外试验Key words
Tibetan medicine/Porcine rotavirus/antivirus/in vitro test分类
农业科技引用本文复制引用
豆薇,李生庆,郭抗抗,赵龙,郝峰,吕长荣,王栋,陈晨,高海慧,韩生义,顾庆云..体外抗猪轮状病毒8种藏药的初步筛选[J].动物医学进展,2024,45(5):18-24,7.基金项目
青海省科技计划项目(2022-NK-118) （2022-NK-118）
西藏自治区科技计划项目(XZ202101YD001C) （XZ202101YD001C）
