动物医学进展2024，Vol.45Issue(5)：25-29,5.
重组pET30a-EgM9蛋白高免兔血清对体外培养细粒棘球绦虫发育的影响
Effects of High Immune Rabbit Serum against Recombinant pET30a-EgM9 Protein on Development of Echinococcus granulosus in Vitro
摘要
Abstract
To explore the regulatory mechanism of EgM9 gene during sexual maturation of Echinococcus granulosus,pET30a-EgM9 recombinant protein high immune serum was used to interfere with Echinococcus granulosus in vitro,and the development of Echinococcus granulosus was observed after intervention.Rabbits were immunized with pET30a-EgM9 protein to obtain high-valence serum.In vitro,the serum was applied to the worms,which was cultured for 30 days.Western blot was used to detect the immunoreactivity of the EgM9 proteins.RT-qPCR was used to analyze the expression level of EgM9 gene.The microscopical observation showed that the worm developed obviously in the direction of cyst formation after intervention with high immune serum.The Western blot showed that the serum of rabbits could form complex with worm protein,and the indirect method showed that the mouse serum of the EgM9 protein did not bind to the above complexes specifically.RT-qPCR analysis showed that the rabbit serum against EgM9 protein could stimulate the expression of EgM9 gene in worms.The expression of EgM9 gene can be promoted by in vitro intervention with high immune serum,which provided a theoretical basis for screening the candidate genes of the final host vaccine of Echinococcus granulosus.关键词
细粒棘球绦虫/体外培养/体外干预Key words
Echinococcus granulosus/in vitro culture/in vitro intervention分类
农业科技引用本文复制引用
余婉蓉,赵莉,张壮志,班万里,王冰洁,潘星羽,幕永辉,刘帅,吾力江·卡马力,徐晶,喻昌盛..重组pET30a-EgM9蛋白高免兔血清对体外培养细粒棘球绦虫发育的影响[J].动物医学进展,2024,45(5):25-29,5.基金项目
新疆维吾尔自治区自然科学基金资助项目(2022D01A279) （2022D01A279）
新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目(KY2022011) （KY2022011）
