重组pET30a-EgM9蛋白高免兔血清对体外培养细粒棘球绦虫发育的影响OA北大核心CSTPCD

To explore the regulatory mechanism of EgM9 gene during sexual maturation of Echinococcus granulosus,pET30a-EgM9 recombinant protein high immune serum was used to interfere with Echinococcus granulosus in vitro,and the development of Echinococcus granulosus was observed after intervention.Rabbits were immunized with pET30a-EgM9 protein to obtain high-valence serum.In vitro,the serum was applied to the worms,which was cultured for 30 days.Western blot was used to detect the immunoreactivity of the EgM9 proteins.RT-qPCR was used to analyze the expression level of EgM9 gene.The microscopical observation showed that the worm developed obviously in the direction of cyst formation after intervention with high immune serum.The Western blot showed that the serum of rabbits could form complex with worm protein,and the indirect method showed that the mouse serum of the EgM9 protein did not bind to the above complexes specifically.RT-qPCR analysis showed that the rabbit serum against EgM9 protein could stimulate the expression of EgM9 gene in worms.The expression of EgM9 gene can be promoted by in vitro intervention with high immune serum,which provided a theoretical basis for screening the candidate genes of the final host vaccine of Echinococcus granulosus.