动物医学进展2024，Vol.45Issue(5)：46-50,5.
妊娠期小鼠血浆促肾上腺皮质激素释放激素水平变化研究
Study on Changes of Plasma CRH Levels in Pregnant Mice
摘要
Abstract
In order to investigate the changes of plasma concentrations of corticotrophin-releasing hormone(CRH)in pregnancy and its relationship with the duration of pregnancy in mice,the female mice were selected for mating naturally,from the day of vaginal suppositories was seen,the plasma was separated and collected,and CRH content was determined by enzyme-linked immunosorbent assay.By analyzing the plasma CRH levels of pregnant mice during pregnancy,delivery day and 24 hours after delivery,it was found that the CRH levels in the plasma of normal pregnant mice increased in the early stages of pregnancy.The plasma CRH level[(21.6±3.90)pg/mL]showed a progressive increase starting from 13.5 days of pregnancy,with a significant increase in late pregnancy and reached its highest level at delivery.CRH content basically returned to normal values 24 hours after delivery.The CRH content of preterm pregnant mice at 13.5 days was higher than that of normal pregnant mice,while the CRH content of dystocia pregnancy mice was significantly lower than that of normal pregnancy mice.The CRH content in the plasma of pregnant mice at 13.5 days was negatively correlated with the duration of pregnancy.These results suggest that the levels of CRH in mouse plasma can be used as a marker to determine the duration of pregnancy,to predict the delivery time of pregnant mice,and providing a theoretical basis for biological purification of cesarean section in mice.关键词
促肾上腺皮质激素释放激素/分娩/妊娠/生物净化Key words
corticotrophin-releasing hormone(CRH)/delivery/pregnancy/biological purification分类
农业科技引用本文复制引用
张曼,彭丽娜,王朝霞..妊娠期小鼠血浆促肾上腺皮质激素释放激素水平变化研究[J].动物医学进展,2024,45(5):46-50,5.基金项目
上海市兽医生物技术重点实验室开放课题项目(SHKLAB202009) （SHKLAB202009）
