动物医学进展2024，Vol.45Issue(5)：56-60,5.
洛那法尼对动物源丁型肝炎病毒作用的初步研究
Possible Effect of Lonafarnib on Animal-derived Hepatitis D Like Virus
摘要
Abstract
The aim of the study is to investigate the effect of Lonafarnib(LNF)on the animal-derived hepatitis D virus(HDV),which will provide insights of animal-derived HDV like virus.We modified HDV infectious clone to recapitulate the key features of animal HDV like virus by using site-directed mutagenesis(SDM),followed by transfection and antibiotic screening.Virus replication and HDAg expression were detected by Western blot and indirect immunofluorescence assay.In addition,the effect of LNF on animal HDV replication was detected by real-time quantitative PCR.The results showed that the modified HDV clones can replicate efficiently in vitro,representing a proper model to study animal HDV like virus.Compared with the untreated group,LNF treatment increased viral RNA levels in HDV model,while no significant effect on the modified HDV clones.This implies that the life cycle and drug responses of animal-derived HDV like viruses are distinct compared with HDV.关键词
丁型肝炎病毒/动物源丁型肝炎病毒/洛那法尼/病毒复制Key words
Hepatitis D virus/animal HDV like virus/lonafarnib/viral replication分类
农业科技引用本文复制引用
李球棣,丁奕博,郭虹波,王文世..洛那法尼对动物源丁型肝炎病毒作用的初步研究[J].动物医学进展,2024,45(5):56-60,5.基金项目
江苏省自然科学基金青年基金项目(BK20210899,BK20210900) （BK20210899,BK20210900）
江苏省卫健委重点项目(ZD2021036) （ZD2021036）
