动物医学进展2024，Vol.45Issue(5)：68-74,7.
烯丙孕素缓释微球的制备及特性研究
Preparation and Characterization of Altrenogest Sustained-release Microspheres
摘要
Abstract
In order to improve the bioavailability of altrenogest,reduce the times of administration and reduce the breeding cost of pig farm,this experiment aimed to prepare altrenogest sustained-release microspheres with long-term performance.The altrenogest sustained-release microspheres were prepared by emulsification-solvent volatilization method using monmethoxy-polyethylene glycol polylactic acid copolymer(mPEG-PLA)as the carrier material.The release of the altrenogest sustained-release microspheres in vitro was measured in the dissolution medium simulating the gastrointestinal tract envi-ronment,and the drug stability of the altrenogest sustained-release microspheres was investigated.The re-sults showed that the optimal preparation conditions of altrenogest sustained-release microspheres were as follows:stirring speed 1000 r/min,Tween-80 content 0.2％,mPEG-PLA concentration 0.07 g/mL,oil-water volume ratio 0.6,gelatin content 1.0％in aqueous phase.Under the optimum preparation condi-tions,the average encapsulation rate of altrenogest sustained-release microspheres was(95.64±0.23)％and the average drug loading was(9.83±0.61)％.mPEG-PLA had the best embedding effect on altrenogest.The altrenogest sustained-release microspheres were spherical or nearly spherical in appearance,with an average particle size of 82.3 µm.Altrenogest sustained-release microspheres can be released continuously for more than 4 h in three different dissolution media containing 1％Tween-80 pH 1.2,pH 4.1 and pH 6.8,respectively,and the release mechanism conforms to the first-order kinetic equation.The stability of altrenogest sustained-release microspheres in high humidity environment was good,but when stored under high temperature and strong light exposure for 10 days,the content of altrenogest decreased by 3.00％and 11.92％respectively.Altrenogest sustained-release microspheres have the properties of sustained-release,and the preparation method is simple,the encapsulation rate is high,the drug loading is good,and it is easy to be industrialized,which can further promote the development and utilization of altrenogest in pig industry.关键词
烯丙孕素/缓释微球/mPEG-PLA/体外释放/药物稳定性Key words
altrenogest/sustained-release microspheres/mPEG-PLA/in vitro release/drug stability分类
农业科技引用本文复制引用
陈沃俊,刘璨颖,张晖,白银山,王丙云,张志远,邓翔云,张妙齐,曾蔼宁,冼伟杭,陈胜锋,陈志胜,计慧琴..烯丙孕素缓释微球的制备及特性研究[J].动物医学进展,2024,45(5):68-74,7.基金项目
广东省普通高校动物干细胞工程技术研究中心项目(2021GCZX006) （2021GCZX006）
