文山高峰牛朊蛋白基因(PRNP)23bp和12bp插入/缺失多态性分析研究OA北大核心CSTPCD

To investigate the polymorphisms of the promoter region 23 bp and the first intron region 12 bp in-sertion/deletion of Wenshan cattle prion protein gene(PRNP),the polymorphism data of PRNP gene 23 bp and 12 bp of Wenshan cattle were genotyped by polymerase chain reaction and agarose gel electrophoresis.Through comparative analysis with the PRNP data of cattle reported in the literature,the resistance of Wenshan cattle to mad cow disease was obtained.Results showed the frequency of the 23 bp allele in the promoter region of the PRNP gene of Wenshan cattle is predominantly represented by the deletion type(0.654).The frequencies of the 12 bp insertion and deletion alleles in the first intron region are 0.913 and 0.087,respectively,and the frequency of the deletion haplotype is very low(0.082).A significant difference(P＜0.05)was observed when comparing the frequencies of the 12 bp insertion/deletion alleles in Wenshan cattle with those reported in cases of BSE-affected and healthy cattle from Germany,the United Kingdom,and Switzerland.However,no significant difference(P＞0.05)was found in the insertion/deletion allele frequencies between Wenshan cattle and water buffalo.Therefore,it is concluded that the relatively high frequency of the 12 bp insertion allele and the extremely low frequency of the deletion type haplotype(23 bp insertion-12 bp deletion)in Wenshan cattle confer a higher resistance to BSE disease,making them a valuable resource for disease-resistant breeding programs.Analysis of disease resistance in local cattle breeds in China is beneficial for providing data support for mo-lecular breeding of disease resistance in cattle breeds.