检验医学与临床2024，Vol.21Issue(9)：1198-1202,1207,6.DOI:10.3969/j.issn.1672-9455.2024.09.003
微小RNA-1246在结直肠癌患者血清中的表达水平及其临床意义
Expression of serum microRNA-1246 in patients with colorectal cancer and its clinical significance
摘要
Abstract
Objective To explore the expression of serum microRNA(miRNA)-1246 in the patients with colorectal cancer(CRC)and its relationship with the clinicopathologic features of CRC patients,and analyze the diagnostic value of miR-1246 combined with carcinoembryonic antigen(CEA)and carbohydrate antigen 199(CA-199)for CRC.Methods Sixty CRC patients who received surgical treatment in Xinmi City Hospital of Traditional Chinese Medicine from January 2017 to December 2020 were selected as the CRC group,and an-other 60 colorectal adenoma(CRA)patients who were hospitalized in this hospital during the same period were selected as the CRA group and 60 healthy subjects who took physical examination in this hospital during the same period as the healthy control group.Serum miR-1246 expression level was detected by real-time fluo-rescence quantitative PCR,serum CEA and CA199 levels were detected by chemiluminescence assay,the cor-relation between serum miR-1246 expression level and serum CEA and CA199 levels was analyzed by Spearman's correlation,and receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of miR-1246 combined with CEA,CA-199 for CRC.Results The serum miR-1246 expression level in the CRC group was significantly higher than that in the CRA group and the healthy control group(P<0.05),whereas the difference of serum miR-1246 expression level between the CRA group and the healthy control group was not statistically significant(P>0.05).The serum miR-1246 expression level in CRC patients with TNM staging Ⅲ-Ⅳ,with lymph node metastasis,and with distant metastasis were significantly higher than those with TNM stage Ⅰ-Ⅱ,no lymph node metastasis and no distant metastasis(P<0.05).Spearman cor-relation analysis showed that miR-1246 expression level had significant positive correlation with the CEA level and CA199 level(r=0.397,P=0.002;r=0.443,P<0.05).The AUC of miR-1246 for diagnosing CRC alone was 0.814,with the specificity of 81.7%and sensitivity of 70.2%,respectively,while the AUC of miR-1246 combined with CEA and CA199 for diagnosing CRC could increased to 0.873,with the specificity of 86.7%and the sensitivity of 74.2%.Conclusion miR-1246 is highly expressed in the serum of CRC patients,which is correlated with TNM staging,lymph node metastasis and distant metastasis,and miR-1246 combined with CEA and CA199 detection can improve the diagnostic efficacy of CRC.关键词
结直肠癌/微小RNA-1246/癌胚抗原/糖类抗原199/临床病理特征/诊断Key words
colorectal cancer/miR-1246/carcinoembryonic antigen/carbohydrate antigen 199/clinicopathologic feature/diagnosis分类
医药卫生引用本文复制引用
刘丽萍,崔发财..微小RNA-1246在结直肠癌患者血清中的表达水平及其临床意义[J].检验医学与临床,2024,21(9):1198-1202,1207,6.基金项目
国家自然科学基金项目(81802094). （81802094）
