血清外泌体miRNA-21、miRNA-214联合检测在骨质疏松症诊断中的价值OACSTPCD
Value of combined detection of serum exosomal miRNA-21 and miRNA-214 in the diagnosis of osteoporosis
目的 探讨骨质疏松症(OP)患者血清外泌体中微小 RNA(miRNA)-21 和 miRNA-214 的相对表达水平及其对 OP的诊断价值.方法 选取重庆市人民医院 2022 年 1 月至 2023 年 12 月收治的 77 例 OP 患者作为OP组,另选取同期该院 81 例健康体检者为健康对照组.分离并鉴定血清外泌体,提取外泌体miRNA.采用实时荧光定量反转录-聚合酶链反应(RT-qPCR)检测两组血清外泌体中miRNA-21 和miRNA-214 的相对表达水平.采用受试者工作特征(ROC)曲线评估血清外泌体中 miRNA-21 和 miRNA-214 对 OP的诊断价值.结果 分离的血清外泌体中CD63 标志蛋白呈高表达.OP组血清外泌体 miRNA-21 和 miRNA-214 相对表达水平分别为 5.302(2.407,9.765)和 1.875(1.605,2.450),明显高于对照组的 1.338(0.801,2.088)和 1.163(0.860,1.391),差异均有统计学意义(P<0.05).多因素 Logistic 回归分析结果显示,血清外泌体 miRNA-21、miRNA-214 相对表达水平升高是导致 OP的危险因素(P<0.05).ROC曲线结果显示,血清外泌体 miR-NA-21、miRNA-214 诊断 OP 的 AUC 分别为 0.853、0.811,灵敏度分别为 80.5%、89.6%,特异度分别为79.0%、82.7%,二者联合诊断 OP的AUC为 0.919,灵敏度为 90.9%,特异度为 84.0%.结论 血清外泌体miRNA-21 和 miRNA-214 单独检测对诊断 OP具有一定的价值,二者联合检测可提高诊断的准确性,有临床应用价值.
Objective To investigate the relative expression levels of microRNA-21(miRNA-21)and mi-croRNA-214(miRNA-214)in serum exosomes of patients with osteoporosis(OP)and their diagnostic value in OP.Methods A total of 77 OP patients admitted to Chongqing General Hospital from January 2022 to De-cember 2023 were selected as the OP group,while 81 healthy individuals who underwent physical examina-tions in the hospital during the same period were selected as the healthy control group.Furthermore,serum exosomes were isolated and characterized,and miRNAs were extracted.Real-time quantitative reverse tran-scription polymerase chain reaction(RT-qPCR)was conducted to assess the relative expression levels of miR-NA-21 and miRNA-214 in the serum exosomes in both groups.Receiver operating characteristic(ROC)curves were employed to assess the diagnostic value of miRNA-21 and miRNA-214 in serum exosomes for OP.Results The CD63 marker protein was highly expressed in isolated serum exosomes.The relative expres-sion levels of miRNA-21 and miRNA-214 in serum exosomes in the OP group were 5.302(2.407,9.765)and 1.875(1.605,2.450),respectively,which were significantly higher than those of the healthy control group[1.338(0.801,2.088),1.163(0.860,1.391)],and the differences were all statistically significant(P<0.05).The results of multivariate Logistic regression analysis showed that elevated relative expression levels of miRNA-21 and miRNA-214 in serum exosomal were risk factors for the development of OP(P<0.05).The results of ROC curve showed that the AUCs of serum exosomal miRNA-21 and miRNA-214 for the diag-nosis of OP were 0.853 and 0.811,with the sensitivities of 80.5%and 89.6%,specificity of 79.0%and 82.7%,respectively,and the AUC of the combined detection of miRNA-21 and miRNA-214 for the diagnosis of OP was 0.919,with sensitivity of 90.9%and specificity of 84.0%.Conclusion The detections of serum ex-osomal miRNA-21 and miRNA-214 alone have some value in diagnosing OP,and the combination detection of the indicators can improve the accuracy of diagnosis of OP,with clinical application value.
祁彩虹;李丽;廖璞
西南医科大学临床医学院检验科,四川泸州 646000重庆市人民医院检验科,重庆 401147
临床医学
骨质疏松症血清外泌体miRNA-21miRNA-214诊断
osteoporosisserum exosomesmicroRNA-21microRNA-214combined detectiondiagnosis
《检验医学与临床》 2024 (009)
1203-1207 / 5
国家自然科学基金青年基金项目(82002257);重庆市科卫联合面上项目(2022MSXM106).
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