检验医学与临床2024，Vol.21Issue(9)：1203-1207,5.DOI:10.3969/j.issn.1672-9455.2024.09.004
血清外泌体miRNA-21、miRNA-214联合检测在骨质疏松症诊断中的价值
Value of combined detection of serum exosomal miRNA-21 and miRNA-214 in the diagnosis of osteoporosis
摘要
Abstract
Objective To investigate the relative expression levels of microRNA-21(miRNA-21)and mi-croRNA-214(miRNA-214)in serum exosomes of patients with osteoporosis(OP)and their diagnostic value in OP.Methods A total of 77 OP patients admitted to Chongqing General Hospital from January 2022 to De-cember 2023 were selected as the OP group,while 81 healthy individuals who underwent physical examina-tions in the hospital during the same period were selected as the healthy control group.Furthermore,serum exosomes were isolated and characterized,and miRNAs were extracted.Real-time quantitative reverse tran-scription polymerase chain reaction(RT-qPCR)was conducted to assess the relative expression levels of miR-NA-21 and miRNA-214 in the serum exosomes in both groups.Receiver operating characteristic(ROC)curves were employed to assess the diagnostic value of miRNA-21 and miRNA-214 in serum exosomes for OP.Results The CD63 marker protein was highly expressed in isolated serum exosomes.The relative expres-sion levels of miRNA-21 and miRNA-214 in serum exosomes in the OP group were 5.302(2.407,9.765)and 1.875(1.605,2.450),respectively,which were significantly higher than those of the healthy control group[1.338(0.801,2.088),1.163(0.860,1.391)],and the differences were all statistically significant(P<0.05).The results of multivariate Logistic regression analysis showed that elevated relative expression levels of miRNA-21 and miRNA-214 in serum exosomal were risk factors for the development of OP(P<0.05).The results of ROC curve showed that the AUCs of serum exosomal miRNA-21 and miRNA-214 for the diag-nosis of OP were 0.853 and 0.811,with the sensitivities of 80.5%and 89.6%,specificity of 79.0%and 82.7%,respectively,and the AUC of the combined detection of miRNA-21 and miRNA-214 for the diagnosis of OP was 0.919,with sensitivity of 90.9%and specificity of 84.0%.Conclusion The detections of serum ex-osomal miRNA-21 and miRNA-214 alone have some value in diagnosing OP,and the combination detection of the indicators can improve the accuracy of diagnosis of OP,with clinical application value.关键词
骨质疏松症/血清外泌体/miRNA-21/miRNA-214/诊断Key words
osteoporosis/serum exosomes/microRNA-21/microRNA-214/combined detection/diagnosis分类
医药卫生引用本文复制引用
祁彩虹,李丽,廖璞..血清外泌体miRNA-21、miRNA-214联合检测在骨质疏松症诊断中的价值[J].检验医学与临床,2024,21(9):1203-1207,5.基金项目
国家自然科学基金青年基金项目(82002257) （82002257）
重庆市科卫联合面上项目(2022MSXM106). （2022MSXM106）
