检验医学与临床2024，Vol.21Issue(9)：1208-1213,6.DOI:10.3969/j.issn.1672-9455.2024.09.005
葱白提取物通过PPARγ/HO-1途径对动脉粥样硬化大鼠血脂异常和炎症反应的调节作用
Regulatory effect of fistular onion bulb extract on dyslipidemia and inflammatory response in atherosclerotic rats via PPARγ/HO-1 pathway
摘要
Abstract
Objective To explore the regulatory effect of fistular onion bulb extract(FOB)via peroxisome proliferators activated receptor γ (PPAR γ )/heme oxygenase-1(HO-1)pathway on dyslipidemia and inflam-matory reaction in atherosclerosis(AS)rats.Methods Forty SD rats were randomly divided into control group,model group(AS group),FOB group and FOB+GW9662 group,with 10 rats in each group.The con-trol group rats were fed with normal feed,while the other three groups of rats established AS models.The rats in each group were treated with drugs for 4 weeks.The aortic lesions and lipid deposition were observed by HE staining and oil red O staining.The levels of total cholesterol(TC),triacylglycerol(TG),low-density lip-oprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C)were detected by automatic analyzer,and the atherosclerosis index(AI)were calculated.The levels of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)were detected by ELISA assay.The mRNA and protein expression levels of PPAR γ and HO-1 were detected by qRT-PCR and Western blot.Results Compared with the control group,the aortic wall in the AS group was significantly thickened,inflammatory cell infiltration and foam cell accumulation were seen under the intima,and lipid plaque was formed;the pathological changes and lipid plaques in the thoracic aorta of rats in the FOB group were significantly improved compared to those in the AS group.The pathological changes and lipid plaques in the thoracic aorta tissue of rats in the FOB+GW9662 group were obviously worse than those in the FOB group.Compared with the Control group,the serum TC,TG,LDL-C,IL-6,IL-1β,TNF-α levels and AI in the AS group were significantly increased(P<0.05),while the serum HDL-C level,mRNA and protein expression levels of PPAR γ and HO-1 in thoracic aortic tissue were significantly decreased(P<0.05).Compared with the AS group,the serum TC,TG,LDL-C,IL-6,IL-1β,TNF-α levels and AI in the FOB group rats were significantly decreased(P<0.05),while the serum HDL-C level,mRNA and protein expression levels of PPAR γ and HO-1 in thoracic aortic tissue were significantly increased(P<0.05).Compared with the FOB group,the serum TC,TG,LDL-C,IL-6,IL-1β,TNF-α levels and AI in the FOB+GW9662 group were significantly increased(P<0.05),while the serum HDL-C level,mRNA and protein expression levels of PPAR γ and HO-1 in thoracic aortic tissue were significantly decreased(P<0.05).Conclusion FOB may modulate dyslipidemia and inflammatory response by activating PPARγ/HO-1 signaling pathway to alleviate the progression of AS disease in rats.关键词
葱白提取物/动脉粥样硬化/脂质代谢/炎症反应/PPARγ/HO-1信号通路Key words
fistular onion bulb extract/arteriosclerosis/lipid metabolism/inflammatory reaction/PPARγ/HO-1 signaling pathway分类
医药卫生引用本文复制引用
范鸿儒,王栋,张帆,杨力,易春峰,贺立群..葱白提取物通过PPARγ/HO-1途径对动脉粥样硬化大鼠血脂异常和炎症反应的调节作用[J].检验医学与临床,2024,21(9):1208-1213,6.基金项目
湖北省知识创新专项(自然科学基金项目)(2019CFB405). （自然科学基金项目）
