基于TCGA数据集分析LINC00900在甲状腺乳头状癌中的表达及临床意义OACSTPCD

Objective To investigate the expression of long chain non-coding RNA00900(LINC00900)in papillary thyroid carcinoma(PTC),and the relationship between LINC00900 and the clinical characteristics.Methods The Cancer Genome Atlas of America(TCGA)database was utilized to collect PTC data informa-tion,download gene expression profile information and PTC clinical case information information.The expres-sion of LINC00900 between PTC cancer tissues and paracancerous thyroid tissues was compared.The relation-ship between the expression of LINC00900 and clinical characteristics of PTC patients was analyzed.Kaplan-Meier survival curves were plotted to analyze the relationship between LINC00900 expression and survival time of PTC patients.Protein-coding genes(PCGs)associated with LINC00900 were predicted by weighted gene co-expression network analysis(WGCNA),and the screened PCGs were subjected to gene ontology(GO)and Kyoto Encyclopedia of Genomes and Genomes(KEGG)pathway analyses by the Database for An-notation,Visualization and Integrated Discovery(DAVID)online annotation tool.Results The expression level of LINC00900 in PTC cancer tissues was significantly higher than that in normal paracancerous thyroid tissues(P<0.001);the differences of LINC00900 levels in PTC patients with different ages,pathological T stages and histological types were statistically significant(P<0.05);the expression level of LINC00900 in PTC patients≥55 years old was significantly lower than that of PTC patients<55 years(P<0.01);the LINC00900 expression level of PTC patients with T3-T4 stage was significantly lower than that of T1-T2 stage(P<0.01);the LINC00900 expression level of PTC patients with high-cellular subtypes was lower than that of classic,follicular and other types(P<0.01);and the results of the Kaplan-Meier survival curves showed that the survival rate of PTC patients with high-expression of LINC00900 was significantly longer than that of the patients with low-expression of LINC00900(Log-rankχ2=6.691,P<0.05).Functional en-richment analysis showed that LINC00900 might affect the progression and prognosis of PTC through path-ways such as nucleosome assembly,splicing of mRNA,modification of histone H3 methylation,chromosome binding,regulation of histone acetyltransferase regulator activity,regulation of sphingosine-1-phosphate phos-phatase activity,and biosynthesis of mucin-type O-glycans.Conclusion High expression of LINC00900 is a favorable prognostic factor for PTC,and it may be a biomarker in both prognosis and diagnosis for PTC in the future.