甲状腺癌患者BRAF V600E基因突变与民族及临床病理特征的关系OACSTPCD

Objective To explore the relationship between BRAF V600 E gene mutations and nationality and clinicopathologic characteristics of thyroid cancer patients.Methods Three hundred patients with thyroid cancer diagnosed at the Affiliated Hospital of Youjiang Medical College for Nationalities from January 2021 to January 2023 were selected and divided into 150 cases of the Zhuang nationality and 150 cases of Han national-ity according to the patients'nationality,and their pathologic specimens were collected.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect gene mutations at the V600 E locus of the BRAF gene.Results Among the 300 thyroid cancer patients,221 cases were mutated and 79 cases were wild-type.The difference of the gene mutation rate at the V600 E locus of the BRAF gene of patients with thy-roid cancer in the Zhuang nationality and the Han nationality(72.00%vs.75.33%)showed no statistical sig-nificance(P>0.05).The difference of mutation rates at the V600 E locus of the BRAF gene between different genders of the same nationality showed statistical significance(P<0.01);the difference of mutation rates at the V600 E locus of the BRAF gene between different nationalities of the same gender showed statistical signif-icance(P<0.01).The mutation rate of BRAF gene at the V600 E locus in thyroid cancer patients<40 years old was 88.67%,which was significantly higher than that of 58.67%in patients≥40 years old,and the differ-ence was statistically significant(P<0.05);however,the differences of the mutation rates of BRAF gene at the V600 E locus in thyroid cancer patients with different maximum tumor diameters and different numbers of lymph nodes were not statistically significant(P>0.05).There was no statistically significant difference in mutation rates at the V600 E locus of the BRAF gene among patients with different nationalities within the same age range(P>0.05).The difference of mutation rates at the V600 E locus of the BRAF gene between thyroid patients of different nationalities with a maximum tumor diameter of<2 cm had statistically signifi-cant difference(P>0.05),but the difference of mutation rates at the V600 E locus of the BRAF gene between thyroid patients of different nationalities with maximum tumor diameters of 2-4 cm was statistically signifi-cant difference(P=0.001).The difference of mutation rates at the V600 E locus of the BRAF gene among thyroid patients of different nationalities with the same number of lymph nodes showed no statistical signifi-cance(P>0.05).The difference of the mutation rate at the V600 E locus of BRAF gene in papillary thyroid cancer patients with different nationalities was statistically significant(P<0.05),but the differences of muta-tion rate at the V600 E locus of BRAF gene in patients with follicular thyroid cancer and undifferentiated thy-roid cancer in patients with different nationalities were not statistically significant(P>0.05).Conclusion The gene mutation rate at the V600 E locus of the BRAF gene in thyroid cancer patients in Baise City is related to na-tionality,gender,maximum diameter of the tumor and tumor type.