利多卡因通过抑制NETs改善阿霉素引起的心脏毒性OACSTPCD

Objective To verify that lidocaine(LIDO)inhibits neutrophil extracellular traps(NETs)and alleviates doxorubicin(DOX)-induced cardiotoxicity by animal experiments.Methods A total of 30 C57 BL/6 mice were randomly divided into control group,doxorubicin group(DOX group)and lidocaine + doxorubicin group(LIDO+DOX group),with 10 mice in each group.The control group was with single intraperitoneal in-jection of normal saline 1 mL/100 g,the DOX group was with single intraperitoneal injection of DOX 15 mg/kg,LIDO+DOX group was with tail vein injection of LIDO 6 mg/kg and intraperitoneal injection of DOX 15mg/kg after 30 min.The expression levels of serum CK and CK-MB were detected by on the 10th day.En-zyme linked immunosorbent assay(ELISA)was adopted to detect the levels of free DNA(cf-DNA),citrulline Histone 3(Cit H3),myeloperoxidase-DNA(MPO-DNA).Immunofluorescence was uesed to detect the expres-sion of Cit H3 and myeloperoxidase(MPO)in NETs-related markers.Results The serum levels of CK and CK-MB in the DOX group were significantly higher than those in the control group and the LIDO+DOX group(P<0.05).The results of the ELISA assay showed that the levels of MPO-DNA,Cit H3,and cf-DNA in the DOX group and the LIDO+DOX group were significantly higher than those in the control group(P<0.05),but the levels of MPO-DNA,Cit H3 and cf-DNA of mice in the LIDO+DOX group were significantly lower than those in the DOX group(P<0.05).Immunofluorescence assay revealed that the expressions of MPO and Cit H3 in the myocardial tissues of mice in the DOX group were significantly higher than those in the control group and the LIDO+DOX group(P<0.05).Conclusion Lidocaine may alleviate adriamycin-in-duced cardiotoxicity by inhibiting the expression of NETs.