检验医学与临床2024，Vol.21Issue(9)：1307-1311,5.DOI:10.3969/j.issn.1672-9455.2024.09.026
铜蓝蛋白用于糖尿病视网膜病变诊断的临床价值
Clinical value of ceruloplasmin in the diagnosis of diabetic retinopathy
摘要
Abstract
Objective To analyze the clinical value of ceruloplasmin(CP)level as a marker of oxidative stress in the diagnosis of diabetic retinopathy(DR).Methods A total of 196 patients with type 2 diabetes mellitus(T2 DM)admitted to the hospital from January 2016 to December 2020 were enrolled as study sub-jects,which were categorized into patients with T2 DM alone(DM group)and patients with DR(DR group)based on ophthalmologic examination,and 70 patients without diabetes mellitus during the same time period were included as control group.DR patients were divided into mild and moderate non-proliferative DR(NP-DR)with 86 cases,severe NPDR and proliferative DR(PDR)with 32 cases according to dilated fundus pho-tography examination,angiography and International Clinical DR Disease Severity Scale of DR.Atrial fluid and serum CP were measured by Somani and Ambade methods,respectively.Superoxide dismutase(SOD),malon-dialdehyde(MDA)in aqueous humor were measured by enzyme-linked immunosorbent assay(ELISA).Spearman correlation was used to analyze the correlation between serum and aqueous humor CP levels and at-rial oxidative stress indicators in DR patients.The diagnostic value of serum CP level for DR was analyzed by plotting the receiver operating characteristic(ROC)curve of the subjects.Results The serum CP level in the DR group was significantly higher than that in the control group and the DM group(P<0.001).Spearman's correlation analysis showed that serum CP level in DR patients was positively correlated with aqueous humor CP and and MDA levels(r=0.620,P<0.001;r=0.198,P=0.001),and negatively correlated with aqueous humor SOD levels(r=-0.196,P=0.001).Multivariate Logistic regression analysis showed that serum CP level was an independent predictor for the occurrence of DR in T2 DM patients or the progression to severe NPDR/PDR in DR patients(P<0.05).The results of the ROC curve showed that the area under the curve of serum CP level for DR diagnosis,DR progression,and early diagnosis of DR were 0.833(95%CI:0.776-0.889),0.890(95%CI:0.827-0.953)and 0.777(95%CI:0.705-0.848),respectively.Conclusion The level of serum CP is abnormally high in DR patients,and increases with the increase of the severity of DR disease.The de-tection of serum CP level is helpful to the diagnosis of DR or to reflect the degree of disease progression.关键词
铜蓝蛋白/氧化应激/糖尿病视网膜病变/疾病进展/诊断Key words
ceruloplasmin/oxidative stress/diabetic retinopathy/disease progression/diagnosis分类
医药卫生引用本文复制引用
肇莉莉,喻磊,王萍,马为梅..铜蓝蛋白用于糖尿病视网膜病变诊断的临床价值[J].检验医学与临床,2024,21(9):1307-1311,5.基金项目
陕西省社会发展科技攻关项目(2021SF232). （2021SF232）
