江西农业大学学报2024，Vol.46Issue(2)：302-313,12.DOI:10.3724/aauj.2024028
马铃薯PYL5基因对非生物胁迫的响应分析及其启动子的活性鉴定
Response analysis of potato PYL5 gene to abiotic stress and activity identification of its promoter
摘要
Abstract
[Objective]Abscisic acid(ABA),as a stress hormone,plays an important role in plant growth,development,and response to abiotic stresses such as drought and salt.PYR/PYL/RCARs(referred to as"PYL"hereafter)as ABA receptors in various plants,are widely studied.Based on the bioinformatics and expression pattern analysis of potato StPYL5 gene and the identification of its promoter activity,this study provides a basis for further revealing the function of potato StPYL5 and resistance breeding.[Method]In this study,StPYL5 gene was cloned based on transcriptome data.The molecular characteristics of StPYL5 was analyzed using software such as DNAMAN and MEGA.The tissue specificity of the StPYL5 gene and its response to abiotic stress were examined by qPCR.The StPYL5 gene promoter was analyzed using the PlantCARE website,and its activity was identified through transient transformation in tobacco.[Result]The results showed that the full-length of the StPYL5 gene was 534 bp,encoding 177 amino acids.The protein had a molecular weight of 20.19 ku,and the theoretical isoelectric point(pI)was 5.97.Phylogenetic analysis revealed a close relationship between StPYL5 and SpPYL9-like.Tissue specificity analysis showed that the expression pattern of StPYL5 in potato cultivar Qingshu 9(QS9)was the highest in leaves and roots,followed by stems,flowers,and tubers.Expression analysis under different stresses showed that the expression level of StPYL5 in QS9 increased initially and then decreased under drought,low temperature,salt,and ABA stress.The expression of StPYL5 was induced by MeJA and SA.In addition,a 2 000 bp StPYL5 gene promoter was successfully cloned.Tissue staining after transient transformation in tobacco demonstrated that the StPYL5 gene promoter had promoter activity to drive downstream GUS reporter gene expression.[Conclusion]This study comprehensively analyzed the molecular characteristics of the StPYL5 gene and its expression profile under various abiotic stresses.The successful cloning of the active pStPYL5 promoter provides a foundation for further investigating the function of the StPYL5 gene and potato breeding for stress tolerance.关键词
马铃薯/ABA信号通路/StPYL5/基因克隆/非生物胁迫/表达分析Key words
Solanum tuberosum L./ABA signaling pathway/StPYL5/gene cloning/abiotic stress/expression analysis分类
农业科技引用本文复制引用
张春利,白江平,解潇飞,张莹,张锋,孙超,毕真真,刘玉汇,刘震,姚攀锋..马铃薯PYL5基因对非生物胁迫的响应分析及其启动子的活性鉴定[J].江西农业大学学报,2024,46(2):302-313,12.基金项目
国家马铃薯产业技术体系(CARS-09-P10)和甘肃省科技计划项目(23JRRA1414) Project supported by National Potato Industry Technology System(CARS-09-P10)and Science and Technology Program of Gansu Province(23JRRA1414) （CARS-09-P10）
