烹调油烟急性暴露促发脂肪组织炎症反应的分子机制OA北大核心CSTPCD

[Background]Cooking oil fumes are closely related to immune response,and adipose tissue also plays an important role in immune regulation.At present,the biological effect and mechanism of inflammation of adipose tissue induced by oil fume exposure are not clear yet. [Objective]To investigate the inflammatory effect of different exposure duration of cooking fumes on adipose tissue in mice and explore the role of Nod-like receptor pyrin domain 3(NLRP3)/cysteinyl aspartate specific proteinase 1(Caspase 1)/interleukin(IL)-1β signaling pathway. [Methods]Forty 8-week-old female C57BL/6J mice were randomly divided into 3-day control group(CON3 group),7-day control group(CON7 group),3-day oil fume exposure group(COF3 group),and 7-day oil fume exposure group(COF7 group),with 10 mice in each group.The mice were exposed to oil fumes in a cooking oil fume formation and exposure equipment(COFFEE)for 20 min,followed by a 10-min pause,1 h a day for consecutive 3 d or 7 d.General condition of mice was observed and body weight was measured every day.After ex-posure,blood was sampled from the eyeball.Serum levels of IL-6,IL-27,and IL-1β were detected by enzyme-linked immunosorbent assay(ELISA).The adipose tissue of mice was collected and observed after hematoxylin-eosin(HE)staining.The percentages of CD4+ and CD8+T cells in adipose tissue were detected by flow cytometry.Real-time quantitative PCR(RT-qPCR)was used to detect the expression levels of nuclear factor-κB(NF-κB),NLRP3,Caspase 1,and IL-1β in adipose tissue.Western blot was used to detect the expression levels of NLRP3,Caspase 1,and IL-1β in adipose. [Results]Compared with the corresponding control group,serum IL-6,IL-27,and IL-1β contents in the COF3 group and the COF7 group were significantly increased(P<0.05)except IL-6 in the COF3 group,and the levels in the COF7 group were significantly higher than those in the COF3 group(P<0.05).Vacuolar lipid droplets in adipocytes decreased,cytoplasm shrank,and inflammatory cells infiltrated in the COF7 group after HE staining.The flow cytometry results showed that the proportions of CD4+ and CD8+T cells in adipocytes of the COF3 group and the COF7 group were increased compared to the corresponding control group,with a significant increase in the COF7 group(P<0.05),and the CD4+/CD8+T ratio also significantly increased progressively in the two groups(P<0.05).The results of RT-qPCR showed that compared with the corresponding control group,the mRNA expression levels of NF-κB,NLRP3,Caspase 1,and IL-1β in adipose tissue of mice in the COF3 group and the COF7 group were significantly increased(P<0.05,P<0.01).The mRNA expression levels of mice in each exposure group gradually increased over time.The Western blot results showed that compared with the corresponding control group,the protein expressions of NLRP3 and Caspase 1 in the COF3 group were significantly increased(P<0.01),and the expression of IL-1β protein also increased but without statistical significance.The protein expressions of NLRP3,Caspase 1,and IL-1β in the COF7 group were significantly higher than those in the CON7 group(P<0.05,P<0.01). [Conclusion]Acute exposure to cooking oil fumes can induce significant inflammatory response in adipose tissue,and the effect gradually increases with the extension of exposure time.The mechanism of action may be related to the activation of NLRP3 inflammasome signaling pathway.